Visualization of Cell Membrane Tension Regulated by the Microfilaments as a "Shock Absorber" in Micropatterned Cells

• Published in: Biology (Basel, Switzerland) Vol. 12; no. 6; p. 889
• Main Authors: Wang, Xianmeng, Li, Na, Zhang, Zhengyao, Qin, Kairong, Zhang, Hangyu, Shao, Shuai, Liu, Bo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-06-2023; MDPI
• Subjects: Actin; Airway management; Biosensors; cell membrane tension; Cell membranes; Cell size; Cells; Cytoskeleton; Dimethylpolysiloxane; Entropy; Extracellular matrix; Filaments; focal adhesion; FRET; Gene expression; macropattern; microfilament; Microfilaments; Morphology; Muscle proteins; Plasmids; Polydimethylsiloxane; Proteins; Statistical analysis; China; United States > US; focal adhesion; microfilament; macropattern; FRET; cell membrane tension
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology12060889

The extracellular stress signal transmits along the cell membrane-cytoskeleton-focal adhesions (FAs) complex, regulating the cell function through membrane tension. However, the mechanism of the complex regulating membrane tension is still unclear. This study designed polydimethylsiloxane stamps with specific shapes to change the actin filaments' arrangement and FAs' distribution artificially in live cells, visualized the membrane tension in real time, and introduced the concept of information entropy to describe the order degree of the actin filaments and plasma membrane tension. The results showed that the actin filaments' arrangement and FAs' distribution in the patterned cells were changed significantly. The hypertonic solution resulted in the plasma membrane tension of the pattern cell changing more evenly and slowly in the zone rich in cytoskeletal filaments than in the zone lacking filaments. In addition, the membrane tension changed less in the adhesive area than in the non-adhesive area when destroying the cytoskeletal microfilaments. This suggested that patterned cells accumulated more actin filaments in the zone where FAs were difficult to generate to maintain the stability of the overall membrane tension. The actin filaments act as shock absorbers to cushion the alternation in membrane tension without changing the final value of membrane tension.