Enhancement of In Vitro Capillary Tube Formation by Substrate Nanotopography
Endothelial progenitor cells are cultured on nanotopographic substrates with a line‐grating geometry and respond to these substrates through aligned and elongated morphology, reduced proliferation, and increased migration relative to flat substrates. The cells also form supercellular band structures...
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| Published in: | Advanced materials (Weinheim) Vol. 20; no. 1; pp. 99 - 103 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Weinheim
WILEY-VCH Verlag
01-01-2008
WILEY‐VCH Verlag |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Endothelial progenitor cells are cultured on nanotopographic substrates with a line‐grating geometry and respond to these substrates through aligned and elongated morphology, reduced proliferation, and increased migration relative to flat substrates. The cells also form supercellular band structures, which lead to enhanced capillary tube formation upon the addition of matrigel. These results suggest that substrate nanotopography can be used to create organized vascular structures in vitro. Image taken at 250 × magnification. |
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| Bibliography: | ark:/67375/WNG-K3LVTWKC-F The authors would like to acknowledge the following: Dr. Eliza Vasile from the Center for Cancer Research, Microscopy and Imaging Core Facility at MIT for assistance with imaging, the Juvenile Diabetes Research Foundation (fellowship to S.G.), the MEMS Technology Group at the Draper Laboratory for direct funding for C.J.B. and use of facilities; funding provided through DL-H-550154; NIH grants R01-DE-013023-06, P41 EB002520-01A1 and 1R01HL076485-01A2. The content of this paper does not necessarily reflect the position or the policy of the government, and no official endorsement should be inferred. Supporting Information is available online from Wiley InterScience or from the authors. MEMS Technology Group - No. DL-H-550154 ArticleID:ADMA200702487 Juvenile Diabetes Research Foundation NIH - No. R01-DE-013023-06; No. P41 EB002520-01A1; No. 1R01HL076485-01A2 istex:46BC3FEE17563A22BEB58CE954C53A85C7D32919 The authors would like to acknowledge the following: Dr. Eliza Vasile from the Center for Cancer Research, Microscopy and Imaging Core Facility at MIT for assistance with imaging, the Juvenile Diabetes Research Foundation (fellowship to S.G.), the MEMS Technology Group at the Draper Laboratory for direct funding for C.J.B. and use of facilities; funding provided through DL‐H‐550154; NIH grants R01‐DE‐013023‐06, P41 EB002520‐01A1 and 1R01HL076485‐01A2. The content of this paper does not necessarily reflect the position or the policy of the government, and no official endorsement should be inferred. Supporting Information is available online from Wiley InterScience or from the authors. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
| ISSN: | 0935-9648 1521-4095 |
| DOI: | 10.1002/adma.200702487 |