Different mechanisms of Trichoderma virens‐mediated resistance in tomato against Fusarium wilt involve the jasmonic and salicylic acid pathways

• Published in: Molecular plant pathology Vol. 19; no. 4; pp. 870 - 882
• Main Authors: Jogaiah, Sudisha, Abdelrahman, Mostafa, Tran, Lam‐Son Phan, Ito, Shin‐Ichi
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01-04-2018; John Wiley and Sons Inc
• Subjects: Barley; Biological control; Cascades; Cell culture; Cyclopentanes - metabolism; defence marker genes; Deficient mutant; Filtrate; Fusarium; Fusarium - pathogenicity; Fusarium oxysporum; Gene expression; Gene Expression Regulation, Plant - physiology; Incidence; induced systemic resistance; Jasmonic acid; Lycopersicon esculentum - metabolism; Lycopersicon esculentum - microbiology; Original; Oxylipins - metabolism; Pathogenesis; Pathogens; Plant Diseases - microbiology; Salicylic acid; Salicylic Acid - metabolism; Seedlings; Signal transduction; Signal Transduction - physiology; Signaling; Solanum lycopersicum; Spores; tomato Fusarium wilt; Tomatoes; Trichoderma - pathogenicity; Trichoderma virens; Wilt; induced systemic resistance; defence marker genes; salicylic acid; tomato Fusarium wilt; Trichoderma virens; jasmonic acid
• ISSN: 1464-6722; 1364-3703
• DOI: 10.1111/mpp.12571

Summary
In the present study, we investigated the role of Trichoderma virens (TriV_JSB100) spores or cell‐free culture filtrate in the regulation of growth and activation of the defence responses of tomato (Solanum lycopersicum) plants against Fusarium oxysporum f. sp. lycopersici by the development of a biocontrol–plant–pathogen interaction system. Two‐week‐old tomato seedlings primed with TriV_JSB100 spores cultured on barley grains (BGS) or with cell‐free culture filtrate (CF) were inoculated with Fusarium pathogen under glasshouse conditions; this resulted in significantly lower disease incidence in tomato Oogata‐Fukuju plants treated with BGS than in those treated with CF. To dissect the pathways associated with this response, jasmonic acid (JA) and salicylic acid (SA) signalling in BGS‐ and CF‐induced resistance was evaluated using JA‐ and SA‐impaired tomato lines. We observed that JA‐deficient mutant def1 plants were susceptible to Fusarium pathogen when they were treated with BGS. However, wild‐type (WT) BGS‐treated tomato plants showed a higher JA level and significantly lower disease incidence. SA‐deficient mutant NahG plants treated with CF were also found to be susceptible to Fusarium pathogen and displayed low SA levels, whereas WT CF‐treated tomato plants exhibited moderately lower disease levels and substantially higher SA levels. Expression of the JA‐responsive defensin gene PDF1 was induced in WT tomato plants treated with BGS, whereas the SA‐inducible pathogenesis‐related protein 1 acidic (PR1a) gene was up‐regulated in WT tomato plants treated with CF. These results suggest that TriV_JSB100 BGS and CF differentially induce JA and SA signalling cascades for the elicitation of Fusarium oxysporum resistance in tomato.