Joint toxicity of tetracycline with copper(II) and cadmium(II) to Vibrio fischeri: effect of complexation reaction

• Published in: Ecotoxicology (London) Vol. 24; no. 2; pp. 346 - 355
• Main Authors: Tong, Fei, Zhao, Yanping, Gu, Xueyuan, Gu, Cheng, Lee, Charles C.C.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-03-2015; Springer Nature B.V
• Subjects: Aliivibrio fischeri - drug effects; Anti-Bacterial Agents - pharmacology; Bacteria; Benzopyrans - metabolism; Cadmium; Cadmium - toxicity; Cations; Citric Acid - metabolism; Complexation; Copper; Copper - toxicity; Earth and Environmental Science; Ecology; Ecotoxicology; Environment; Environmental Management; Fulvic acids; Heavy metals; Ligands; Mathematical models; Prediction models; Speciation; Synergistic effect; Tetracycline - pharmacology; Tetracyclines; Toxicity; Toxicity Tests - methods; Vibrio; Vibrio fischeri; Cadmium; Copper; Joint toxicity; Tetracycline
• ISSN: 0963-9292; 1573-3017
• DOI: 10.1007/s10646-014-1383-7

Co-contamination of antibiotic and heavy metals commonly occurs in the environment. Tetracycline (TC), a common antibiotic, can behave as an efficient organic ligand to complex with cations. In this paper, the joint toxicity of TC with two commonly existing metals, copper(II) and cadmium(II), towards a luminescent bacteria,
Vibrio fischeri,
are investigated. Results showed that coexistence of TC and Cu(II) showed a significant antagonistic effect, while TC and Cd(II) showed a synergistic effect. The aqueous speciation of TC with two metal cations was calculated using a chemical equilibrium software Visual MINTEQ and results indicated that a strong complexation exist between TC and Cu(II), while much weaker interaction between TC and Cd(II). Traditional joint toxicity prediction model based on independent action failed to predict the combined toxicity of TC with metals. A new method based on speciation calculation was used to evaluate the joint toxicity of ligands and cations. It is assumed that the metal–ligand complexes are non-toxic to
V. fischeri
and the joint toxicity is determined by the sum of toxic unit of free metal-ions and free organic ligands. It explained the joint toxicity of the mixed systems reasonably well. Meanwhile, citric acid (CA) and fulvic acid (FA) were also introduced in this study to provide a benchmark comparison with TC. Results showed it is also valid for mixed systems of CA and FA with metals except for the Cd–CA mixture.