Continued sustained release of VEGF by PLGA nanospheres modified BAMG stent for the anterior urethral reconstruction of rabbit

• Published in: Asian Pacific journal of tropical medicine Vol. 6; no. 6; p. 481
• Main Authors: Wang, Ji-Hong, Xu, Yue-Min, Fu, Qiang, Song, Lu-Jie, Li, Chao, Zhang, Qin, Xie, Ming-Kai
• Format: Journal Article
• Language: English
• Published: India 01-06-2013
• Subjects: Analysis of Variance; Animals; Antigens, CD34 - metabolism; Delayed-Action Preparations; Gene Expression - drug effects; Immunohistochemistry; Lactic Acid - administration & dosage; Lactic Acid - chemistry; Male; Materials Testing; Nanospheres - administration & dosage; Nanospheres - chemistry; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism; Polyglycolic Acid - administration & dosage; Polyglycolic Acid - chemistry; Rabbits; Reconstructive Surgical Procedures - instrumentation; Reconstructive Surgical Procedures - methods; Stents; Tissue Scaffolds - chemistry; Urethra - surgery; Urinary Bladder - surgery; Vascular Endothelial Growth Factor A - administration & dosage; Vascular Endothelial Growth Factor A - chemistry; Vascular Endothelial Growth Factor A - pharmacokinetics
• ISSN: 2352-4146
• DOI: 10.1016/S1995-7645(13)60078-4

To study the biocompatibility and neovascularization of the PLGA nanospheres wrapped with vascular endothelial growth factor (VEGF), which can improve bladder acellular matrix graft (BAMG) with local continuous release of VEGF.
A total of 18 rabbit model (length of stenosis: 3 cm) with anterior urethral stricture were used as experimental animals and divided into three groups. Group A as the control group: Simple BAMG scaffold materials for urethral reconstruction. Group B as the blank group: PLGA microspheres modified BAMG for urethral reconstruction. Group C: PLGA conjugated with VEGF and modified BAMG for the urethral reconstruction. All rabbits underwent urethral angiography after 7 days, 15 days, 1 month and 3 months after the operation, and one rabbit in each group was sacrificed to be prepared for the organization histologic examination, HE staining, masson staining, CD31, 34 and a-SAM immunohistochemical detection in the repaired sites.
In group A, significant urethral restenosis occurred in two rabbits after 15 days of the operation, HE and masson staining showed a lot of collagen arranged in the repaired sites, and there were a large number of inflammatory cell infiltration, and there were also CD31, 34 in the repaired sites. a-SAM microvascular tag count showed a small amount of microvascular; Group B showed anastomotic restenosis, HE and masoon staining showed inflammatory cell infiltration and collagen deposition; Group C: urethrography showed lumen patency. There were a small amount of inflammatory cell infiltration after 7 and 15 days after the operation, and there were also CD31, 34 in the repaired sites. The a-SAM microvascular tag count showed many microvascular. And the difference was significant.
Anterior urethral reconstruction with sustained-release of VEGF by PLGA nanospheres modified BAMG stents can reduce postoperative restenosis. It can also reduce collagen deposition and scar formation, promote angiogenesis of the repair tissue; therefore it in valuable in the tissue-engineered urethral reconstruction.