Anti-proliferative effect of resveratrol, a natural component of grapes and wine, on human colonic cancer cells

• Published in: Cancer letters Vol. 158; no. 1; pp. 85 - 91
• Main Authors: Schneider, Yann, Vincent, Florence, Duranton, Benoı̂t, Badolo, Lassina, Gossé, Francine, Bergmann, Christian, Seiler, Nikolaus, Raul, Francis
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 29-09-2000; Elsevier
• Subjects: Antineoplastic Agents, Phytogenic - pharmacology; Biological and medical sciences; CaCo-2 cell; Caco-2 Cells; Carcinogenesis, carcinogens and anticarcinogens; Cell cycle; Cell Cycle - drug effects; Cell Division - drug effects; Colonic Neoplasms; Drug Screening Assays, Antitumor; Foods and miscellaneous; Humans; Medical sciences; Ornithine decarboxylase; Ornithine Decarboxylase - metabolism; Ornithine Decarboxylase Inhibitors; Polyamine; Polyamines - metabolism; Polyphenol; Resveratrol; Rosales - chemistry; Stilbenes - pharmacology; Tumors; Wine - analysis; Polyphenol; Polyamine; Ornithine decarboxylase; CaCo-2 cell; Cell cycle; Cell proliferation; Wine; Lyases; Putrescine; Anticarcinogen; Carcinogenesis; Prevention; Enzymatic activity; Carboxy-lyases; Established cell line; Intestinal disease; Colon; Tumor cell; Human; Enzyme; Malignant tumor; Metabolism; In vitro; Biological activity; Colonic disease; Carbon-carbon lyases; Phytoalexin; Grape; Plant origin; Digestive diseases; Intracellular
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(00)00511-5

Resveratrol, a natural polyphenolic phytoalexine present in grapes and wines, has been reported to exert a variety of important pharmacological effects. We investigated the effects of resveratrol on the growth and polyamine metabolism of CaCo-2 human colon cancer cells. Treatment of the CaCo-2 cells with 25 μM resveratrol caused a 70% growth inhibition. The cells accumulated at the S/G2 phase transition of the cell cycle. No signs of cytotoxicity or apoptosis were detected. Resveratrol caused a significant decrease of ornithine decarboxylase (ODC) activity, a key enzyme of polyamine biosynthesis, which is enhanced in cancer growth. ODC inhibition resulted in the reduction of the intracellular putrescine content, indicating that polyamines might represent one of several targets involved in the anti-proliferative effects of resveratrol.