Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium

• Published in: Fertility and sterility Vol. 93; no. 5; pp. 1643 - 1649
• Main Authors: Rossi, Alexandre Guilherme Zabeu, Ph.D, Teixeira Gomes, Regina Célia, Ph.D, de Jesus Simões, Manuel, Ph.D, dos Santos Simões, Ricardo, M.D, Oliveira, Patrícia B., B.S, Soares, José Maria, Ph.D, Baracat, Edmund C., Ph.D
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 15-03-2010; Elsevier
• Subjects: Animals; Biological and medical sciences; Digestive system; Disease Models, Animal; Endocrinopathies; endometrium; Endometrium - drug effects; Endometrium - metabolism; Estradiol - administration & dosage; Estradiol - blood; estrogen; Female; Gene Expression Regulation; Gynecology. Andrology. Obstetrics; Hormone Replacement Therapy; Hyperprolactinemia; Hyperprolactinemia - chemically induced; Hyperprolactinemia - genetics; Hyperprolactinemia - metabolism; Hypothalamus. Hypophysis. Epiphysis (diseases); Internal Medicine; Medical sciences; Metoclopramide; Mice; mouse; Non tumoral diseases. Target tissue resistance. Benign neoplasms; Obstetrics and Gynecology; Ovariectomy; Pharmacology. Drug treatments; Progesterone - administration & dosage; Progesterone - blood; prolactin; prolactin receptor; Protein Isoforms; Receptors, Prolactin - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; prolactin receptor; endometrium; mouse; estrogen; prolactin; Hyperprolactinemia; Endocrinopathy; Dopamine antagonist; Gynecology; Rodentia; Estrogen; Metoclopramide; Obstetrics; Vertebrata; Mammalia; D2 Dopamine receptor; Mouse; Prolactin; Animal; Pituitary diseases; Benzamide derivatives; Antiemetic; Endometrium
• ISSN: 0015-0282; 1556-5653
• DOI: 10.1016/j.fertnstert.2009.02.021

Objective To evaluate the effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium. Design Experimental study using the RNA extraction to detect tissue prolactin receptor isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR). Setting University-based laboratory. Animal(s) Seventy-two female swiss albino mice ( Mus musculus ), approximately 100 days old, were divided into six 12-animal groups: (GI) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (GIII) oophorectomized mice treated with metoclopramide; (GIV) oophorectomized mice treated with metoclopramide and 17β-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17β-estradiol and micronized progesterone. Intervention(s) Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay. Main Outcome Measure(s) Identification of uterine prolactin receptor isoforms. Result(s) The PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide), the two groups with nonoophorectomized animals. The amount of PRL receptor mRNA and that of its isoform L from GII were the largest. No other isoforms of the prolactin receptor were identified in any of the groups. Conclusion(s) Our results suggest that replacement of estrogen and progestin may not increase the mRNA of endometrial PRL receptor in metoclopromide-induced hyperprolactinemia in rats after castration.