Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity

• Published in: Chemico-biological interactions Vol. 171; no. 2; pp. 236 - 249
• Main Authors: Lapidus, Alla, Goltsman, Eugene, Auger, Sandrine, Galleron, Nathalie, Ségurens, Béatrice, Dossat, Carole, Land, Miriam L., Broussolle, Veronique, Brillard, Julien, Guinebretiere, Marie-Helene, Sanchis, Vincent, Nguen-the, Christophe, Lereclus, Didier, Richardson, Paul, Wincker, Patrick, Weissenbach, Jean, Ehrlich, S. Dusko, Sorokin, Alexei
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01-01-2008; Elsevier [1969-....]
• Subjects: BACILLUS; Bacillus anthracis; BACILLUS CEREUS; Bacillus cereus - genetics; Bacillus cytotoxicus; Bacillus cytotoxis; BACTERIA; BASIC BIOLOGICAL SCIENCES; cereus; cereus toxins; FOOD; Food Microbiology; Food poisoning; Foodborne Diseases - microbiology; GENES; Genome, Bacterial; Genomics; Humans; Life Sciences; PATHOGENS; Psychrophily; STRAINS; TOXICITY; cereus toxins; Psychrophily; Bacillus cytotoxis; Genomics; Food poisoning; cereus; Bacillus cytotoxicus; bacillus cytotoxicus; psychrophily; bacillus cytotoxis; genomics; food poisoning
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2007.03.003

The
Bacillus cereus group represents sporulating soil bacteria containing pathogenic strains which may cause diarrheic or emetic food poisoning outbreaks. Multiple locus sequence typing revealed a presence in natural samples of these bacteria of about 30 clonal complexes. Application of genomic methods to this group was however biased due to the major interest for representatives closely related to
Bacillus anthracis. Albeit the most important food-borne pathogens were not yet defined, existing data indicate that they are scattered all over the phylogenetic tree. The preliminary analysis of the sequences of three genomes discussed in this paper narrows down the gaps in our knowledge of the
B. cereus group. The strain NVH391-98 is a rare but particularly severe food-borne pathogen. Sequencing revealed that the strain should be a representative of a novel bacterial species, for which the name
Bacillus cytotoxis or
Bacillus cytotoxicus is proposed. This strain has a reduced genome size compared to other
B. cereus group strains. Genome analysis revealed absence of sigma B factor and the presence of genes encoding diarrheic Nhe toxin, not detected earlier. The strain
B. cereus F837/76 represents a clonal complex close to that of
B. anthracis. Including F837/76, three such
B. cereus strains had been sequenced. Alignment of genomes suggests that
B. anthracis is their common ancestor. Since such strains often emerge from clinical cases, they merit a special attention. The third strain, KBAB4, is a typical facultative psychrophile generally found in soil. Phylogenic studies show that in nature it is the most active group in terms of gene exchange. Genomic sequence revealed high presence of extra-chromosomal genetic material (about 530
kb) that may account for this phenomenon. Genes coding Nhe-like toxin were found on a big plasmid in this strain. This may indicate a potential mechanism of toxicity spread from the psychrophile strain community. The results of this genomic work and ecological compartments of different strains incite to consider a necessity of creating prophylactic vaccines against bacteria closely related to NVH391-98 and F837/76. Presumably developing of such vaccines can be based on the properties of non-pathogenic strains such as KBAB4 or ATCC14579 reported here or earlier. By comparing the protein coding genes of strains being sequenced in this project to others we estimate the shared proteome, or core genome, in the
B. cereus group to be 3000
±
200 genes and the total proteome, or pan-genome, to be 20–25,000 genes.