Identification of genes responsive to BPDE treatment in HeLa cells using cDNA expression assays

• Published in: Environmental and molecular mutagenesis Vol. 36; no. 3; pp. 201 - 205
• Main Authors: Yu, Zhe, Ford, Barry N., Glickman, Barry W.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 2000; Wiley-Liss
• Subjects: 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - toxicity; apoptotic cysteine protease MCH4; Biological and medical sciences; BPDE; Carcinogens - toxicity; cDNA array; Cell physiology; DNA, Complementary - genetics; Effects of physical and chemical agents; fos-related antigen 2; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; genotoxic stress; HeLa cell; HeLa Cells; highly basic protein; hsp27 protein; Humans; Molecular and cellular biology; Molecular genetics; Mutagenesis. Repair; Mutagenicity Tests; NADH-ubiquinone oxidoreductase; Proteins - genetics; ribosomal protein S9; transcription factor ETR103; Transfection; zinc finger protein 91; Human; Carcinogen; Cell line; Gene; Poison; Polycyclic aromatic compound; Gene expression; Hela cell line
• ISSN: 0893-6692; 1098-2280
• DOI: 10.1002/1098-2280(2000)36:3<201::AID-EM3>3.0.CO;2-1

Genotoxic stresses induce cellular responses that can be observed at the level of gene expression. We have studied changes in gene expression following BPDE exposure in HeLa cells by using a cDNA expression array of 597 human genes. After a 53‐hr exposure to 0.4 μM BPDE, nine genes were upregulated. The protein products of these genes are: fos‐related antigen 2, apoptotic cysteine protease MCH4, DB1 (zinc finger protein 91), transcription factor ETR103, integrin alpha, interleukin‐4, interleukin‐6, 23‐kDa highly basic protein, and ribosomal protein S9. We observed the downregulation of gene expression of three genes: heat‐shock protein 27, DNA‐binding protein TAX, and NADH‐ubiquinone oxidoreductase B18 subunit. These results suggest unknown functions or regulatory circuits for several of the responsive genes and demonstrate the complexity of cellular responses to genotoxic insults. Environ. Mol. Mutagen. 36:201–205, 2000 © 2000 Wiley‐Liss, Inc.