Plant-expressed HA as a seasonal influenza vaccine candidate

• Published in: Vaccine Vol. 26; no. 23; pp. 2930 - 2934
• Main Authors: Shoji, Yoko, Chichester, Jessica A, Bi, Hong, Musiychuk, Konstantin, de la Rosa, Patricia, Goldschmidt, Lauren, Horsey, April, Ugulava, Natalia, Palmer, Gene A, Mett, Vadim, Yusibov, Vidadi
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 02-06-2008; Elsevier; Elsevier Limited
• Subjects: Allergy and Immunology; Animals; Antibodies, Viral - biosynthesis; Applied microbiology; Biological and medical sciences; Blotting, Western; Chromatography; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Hemagglutination Inhibition Tests; Hemagglutinin; Hemagglutinins - biosynthesis; Hemagglutinins - immunology; Immunization; Immunodiffusion; Influenza; Influenza Vaccines - biosynthesis; Influenza Vaccines - immunology; Laboratories; Mice; Mice, Inbred BALB C; Microbiology; Neutralization Tests; Nicotiana; Plant-produced vaccine; Plants - genetics; Plants - metabolism; Sheep; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Plant-produced vaccine; Hemagglutinin; Influenza; Infection; Viral disease; Influenza A; Vaccine
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2008.03.045

Abstract Influenza is a globally important respiratory pathogen that causes a high degree of morbidity and mortality annually. Although current vaccines are effective against virus infection, new strategies need to be developed to satisfy the global demand for an influenza vaccine. To address this point, we have engineered and produced the full-length hemagglutinin (HA) protein from the A/Wyoming/03/03 (H3N2) strain of influenza in plants. The antigenicity of this plant-produced HA was confirmed by ELISA and single-radial immunodiffusion (SRID) assays. Immunization of mice with plant-produced HA resulted in HA-specific humoral (IgG1, IgG2a and IgG2b) and cellular (IFNγ and IL-5) immune responses. In addition, significant serum hemagglutination inhibition (HI) and virus neutralizing (VN) antibody titers were obtained with an antigen dose as low as 5 μg. These results demonstrate that plant-produced HA protein is antigenic and can induce immune responses in mice that correlate with protection.