miRNAs in mtDNA-less cell mitochondria

• Published in: Cell death discovery Vol. 1; no. 1; p. 15004
• Main Authors: Dasgupta, N, Peng, Y, Tan, Z, Ciraolo, G, Wang, D, Li, R
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 27-07-2015; Springer Nature B.V; Nature Publishing Group
• Subjects: Apoptosis; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell Cycle Analysis; Life Sciences; Stem Cells
• ISSN: 2058-7716; 2058-7716
• DOI: 10.1038/cddiscovery.2015.4

The novel regulation mechanism in mtDNA-less cells was investigated. Very low mtDNA copy in mtDNA-less 206
ρ
° cells was identified. But no 13 mitochondria-specific proteins were translated in 206
ρ
° cells. Their mitochondrial respiration complexes V, III and II were 86.5, 29.4 and 49.6% of 143B cells, respectively. Complexes I and IV completely lack in 206
ρ
° cells. Non-mitochondrial respiration to generate ATP in 206
ρ
° cells was discovered. The expression levels of some mitochondrial RNAs including 12S rRNA, COX1, COX2, COX3, ND4 and ND5 were low. However, ND1, ND3 and Cyto b were not expressed in 206
ρ
° cells. Unequal transcription of mitochondrial RNAs indicated the post-transcriptional cleavage and processing mechanisms in the regulation of mitochondrial gene expression in 206
ρ
° cells. MicroRNAs (miRNAs) may modulate these mitochondrial RNA expression in these cells. RNA-induced silencing complex indeed within 206
ρ
° cell mitochondria indicated miRNAs in 206
ρ
° cell mitochondria. miRNA profile in mtDNA-less 206
ρ
° cells was studied by next-generation sequencing of small RNAs. Several mitochondria-enriched miRNAs such as miR-181c-5p and miR-146a-5p were identified in 206
ρ
° cell mitochondria. miR-181c-5p and miR-146a-5p had 23 and 19 potential targets on mitochondrial RNAs respectively, and these two miRNAs had multiple targets on mitochondria-associated messenger RNAs encoded by nuclear genes. These data provided the first direct evidence that miRNAs were imported into mitochondria and regulated mitochondrial RNA expressions.