MiR-34b regulates cervical cancer cell proliferation and apoptosis

• Published in: Artificial cells, nanomedicine, and biotechnology Vol. 47; no. 1; pp. 2042 - 2047
• Main Authors: Cao, Zhen, Zhang, Gong, Xie, Conghua, Zhou, Yunfeng
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-12-2019; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Apoptosis; biomarker; Biotechnology; Cancer; Carcinoma; Cell growth; Cell proliferation; Cervical cancer; Cervix; Cholecystokinin; Flow cytometry; miR-34b; Polymerase chain reaction; Reverse transcription; TGF-β1; Transforming growth factor-b1; Tumors; Western blotting; TGF-β1; biomarker; miR-34b; Cervical cancer
• ISSN: 2169-1401; 2169-141X
• DOI: 10.1080/21691401.2019.1614013

MiR-34b is a tumour suppressor in different kinds of carcinomas. This study investigated the role of miR-34b in proliferation and apoptosis of cervical cancer.
The expression of miR-34b in 60 cervical cancer patients were quantified by RT-PCR and correlated with their clinicopathological parameters. Besides, there is a significant reverse relationship between miR-43b and TGF-β1 expression in tumour tissues. Cell proliferation and apoptosis was detected by CCK-8 assays and flow cytometry in cell lines transfected with miR-34b mimics. Western blotting, quantitative reverse transcription-PCR (RT-PCR) and luciferase assays were conducted to analyze the regulation of TGF-β1 by miR-34b in cell lines.
Here, we found expression of miR-34b to be downregulated in cervical cancer in comparison with the adjacent normal tissues. Expression levels of miR-34b were associated with enhanced malignant potential, such as tumour stage and stromal invasion. The overexpression of miR-34b potently suppressed cell proliferation and induced the apoptosis of cell lines.
MiR-34b and TGF-β1 contribute to cervical cancer cell proliferation and apoptosis and are potential targets for cervical cancer therapeutics.