The released interleukin 2 receptor binds interleukin 2 efficiently

• Published in: The Journal of immunology (1950) Vol. 137; no. 12; pp. 3841 - 3844
• Main Authors: Rubin, LA, Jay, G, Nelson, DL
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15-12-1986; American Association of Immunologists
• Subjects: AIDS/HIV; Analysis of the immune response. Humoral and cellular immunity; Biological and medical sciences; Carbohydrates - analysis; Cell Line; Chromatography, Affinity; Deltaretrovirus; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; Interleukin-2 - metabolism; Lymphokines, interleukins ( function, expression); Monocytes - secretion; Receptors, Immunologic - isolation & purification; Receptors, Immunologic - metabolism; Receptors, Interleukin-2; Regulatory factors and their cellular receptors; T-Lymphocytes - secretion; Human; Membrane receptor; Interleukin 2; Molecular interaction; Chemical composition; Glycoproteins; Lymphokine; Release
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.137.12.3841

The released interleukin 2 receptor (IL 2R) molecule was characterized in order to clarify its biochemical structure and to determine its functional capacity. Enzymatic digestions demonstrated that the released IL 2R, like the cell surface IL 2R, is a complex glycoprotein, modified by the addition of both N- and O-linked carbohydrates and sialic acid residues. It has a peptide backbone that is approximately 10 Kd smaller than that of its membrane-associated counterpart. Affinity chromatography demonstrated that released IL 2R from either an HTLV-I-positive T cell line (HUT-102) or PHA-activated normal peripheral lymphocytes binds efficiently to purified recombinant IL 2. Furthermore, the interaction between the growth factor and the released receptor does not appear to require any accessory molecules. These observations suggest a potentially significant role for the released IL 2R in the regulation of IL 2-dependent lymphocyte functions.