The Escherichia coli Gene Encoding the UDP-2,3-diacylglucosamine Pyrophosphatase of Lipid A Biosynthesis

• Published in: The Journal of biological chemistry Vol. 277; no. 29; pp. 25937 - 25946
• Main Authors: Babinski, Kristen J., Ribeiro, Anthony A., Raetz, Christian R.H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 19-07-2002; American Society for Biochemistry and Molecular Biology
• Subjects: Acyltransferases - metabolism; Amidohydrolases - metabolism; Amino Acid Sequence; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli - enzymology; Escherichia coli - genetics; Haemophilus influenzae; Lipid A - biosynthesis; Magnetic Resonance Spectroscopy; Models, Chemical; Molecular Sequence Data; Pyrophosphatases - genetics; Pyrophosphatases - metabolism
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M204067200

UDP-2,3-diacylglucosamine hydrolase is believed to catalyze the fourth step of lipid A biosynthesis inEscherichia coli. This reaction involves pyrophosphate bond hydrolysis of the precursor UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate and UMP. To identify the gene encoding this hydrolase, E. coli lysates generated with individual λ clones of the ordered Kohara library were assayed for overexpression of the enzyme. The sequence of λ clone 157[6E7], promoting overproduction of hydrolase activity, was examined for genes encoding hypothetical proteins of unknown function. The amino acid sequence of one such open reading frame, ybbF, is 50.5% identical to a Haemophilus influenzae hypothetical protein and is also conserved in most other Gram-negative organisms, but is absent in Gram-positives. Cell extracts prepared from cells overexpressing ybbF behind the T7lac promoter have ∼540 times more hydrolase activity than cells with vector alone. YbbF was purified to ∼60% homogeneity, and its catalytic properties were examined. Enzymatic activity is maximal at pH 8 and is inhibited by 0.01% (or more) Triton X-100. The apparent Km for UDP-2,3-diacylglucosamine is 62 μm. YbbF requires a diacylated substrate and does not cleave CDP-diacylglycerol.31P NMR studies of the UMP product generated from UDP-2,3-diacylglucosamine in the presence of 40% H2180 show that the enzyme attacks the α-phosphate group of the UDP moiety. Because ybbF encodes the specific UDP-2,3-diacylglucosamine hydrolase involved in lipid A biosynthesis, it is now designated lpxH.