Identifying active rumen epithelial associated bacteria and archaea in beef cattle divergent in feed efficiency using total RNA-seq

• Published in: Current research in microbial sciences Vol. 2; p. 100064
• Main Authors: Tan, Rebecca S.G., Zhou, Mi, Li, Fuyong, Guan, Le Luo
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-12-2021; Elsevier
• Subjects: 16S rRNA transcripts; Beef cattle; Feed efficiency; Mucosal associated microbiota; Research Paper; Rumen epithelium; Total RNA sequencing; Beef cattle; Rumen epithelium; Feed efficiency; Mucosal associated microbiota; Total RNA sequencing; 16S rRNA transcripts
• ISSN: 2666-5174; 2666-5174
• DOI: 10.1016/j.crmicr.2021.100064

•The most active archaea in the epimural community were different from that of the liquid and content-associated community, but the exact taxonomy requires further identification.•Rumen epithelial attached methanogens may not contribute to differences in CH4 production and variations in feed efficiency.•Families Campylobacteraceae and Neisseriaceae, which contain oxygen scavenging bacteria were significantly more active on the epithelium of efficient cattle.
To date, the role of ruminal epithelial attached microbiota in cattle feed efficiency is undefined. In this study, we aimed to characterize transcriptionally active bacteria and archaea attached to the rumen epithelial wall and to determine whether they differ in cattle with varied feed efficiency. RNA-sequencing was performed to obtain the rumen epithelial transcriptomes from 9 of the most efficient (low RFI) and 9 of the most inefficient (high RFI) animals. The bacteria and archaea 16S rRNA transcripts were identified using an in-house developed pipeline, enriched from filtered reads that did not map to the bovine genome. Archaea from unclassified genera belonging to the Euryarchaeota phylum showed the most activity on the rumen epithelium of low RFI (81.3 ± 1.9%) and high RFI (76.4 ± 3.0%) steers. Bacteria from the Succinivibrionaceae family showed the greatest activity of bacteria on the low RFI (28.7 ± 9.0%) and high RFI (33.9± 8.8%) epithelium. Of the bacterial families, Campylobacteraceae and Neisseriaceae had significantly greater activity on the low RFI epithelium (p < 0.05) and are known to play a role in oxygen scavenging. Greater activity of rumen epithelial attached oxygen scavenging bacteria may provide more optimal feed fermentation conditions, which contributes to high fermentation efficiency in the rumen.