A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese Extracts

• Published in: Nutrients Vol. 13; no. 3; p. 897
• Main Authors: Cardin, Guillaume, Poupet, Cyril, Bonnet, Muriel, Veisseire, Philippe, Ripoche, Isabelle, Chalard, Pierre, Chauder, Anne, Saunier, Etienne, Priam, Julien, Bornes, Stéphanie, Rios, Laurent
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10-03-2021; MDPI
• Subjects: Aging; Animals; Biodiversity; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans - physiology; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Cell Nucleus - metabolism; Cell Survival; Cheese; Chemical fractionation; Chemical Sciences; DAF-16; Dairy products; Ethanol; Fermented food; Food, Preserved; Forkhead Transcription Factors - genetics; Forkhead Transcription Factors - metabolism; Fractionation; Freeze Drying; Gene Expression Regulation; Goat's milk; Insulin; Investigations; Leukocytes; Leukocytes - physiology; Life expectancy; Life span; Lipids; Longevity; MAP kinase; MAP Kinase Kinase 4 - genetics; MAP Kinase Kinase 4 - metabolism; MAP Kinase Signaling System; Metabolites; Microorganisms; Milk; Mitogen-Activated Protein Kinases - genetics; Mitogen-Activated Protein Kinases - metabolism; Mutation; Nematodes; Oxidation resistance; Oxidation-Reduction; Oxidative Stress; p38 MAPK; raw-milk cheese; Reactive Oxygen Species - metabolism; Signal Transduction; Survival; Tetradecanoylphorbol Acetate - pharmacology; Transcription factors; United States > US; Caenorhabditis elegans; longevity; raw-milk cheese; p38 MAPK; oxidative stress; DAF-16
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13030897

Many studies have highlighted the relationship between food and health status, with the aim of improving both disease prevention and life expectancy. Among the different food groups, fermented foods a have huge microbial biodiversity, making them an interesting source of metabolites that could exhibit health benefits. Our previous study highlighted the capacity of raw goat milk cheese, and some of the extracts recovered by the means of chemical fractionation, to increase the longevity of the nematode
. In this article, we pursued the investigation with a view toward understanding the biological mechanisms involved in this phenomenon. Using mutant nematode strains, we evaluated the implication of the insulin-like DAF-2/DAF-16 and the p38 MAPK pathways in the phenomenon of increased longevity and oxidative-stress resistance mechanisms. Our results demonstrated that freeze-dried raw goat milk cheese, and its extracts, induced the activation of the DAF-2/DAF-16 pathway, increasing longevity. Concerning oxidative-stress resistance, all the extracts increased the survival of the worms, but no evidence of the implication of both of the pathways was highlighted, except for the cheese-lipid extract that did seem to require both pathways to improve the survival rate. Simultaneously, the cheese-lipid extract and the dried extract W70, obtained with water, were able to reduce the reactive oxygen species (ROS) production in human leukocytes. This result is in good correlation with the results obtained with the nematode.