Aminopeptidase N/CD13 targeting fluorescent probes: Synthesis and application to tumor cell imaging

• Published in: Peptides (New York, N.Y. : 1980) Vol. 26; no. 11; pp. 2182 - 2187
• Main Authors: Zhang, Zhouen, Harada, Hiroshi, Tanabe, Kazuhito, Hatta, Hiroshi, Hiraoka, Masahiro, Nishimoto, Sei-ichi
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-11-2005; Elsevier Science
• Subjects: Aminopeptidase N (APN/CD13); Biological and medical sciences; CD13 Antigens - chemistry; Cell Line, Tumor; CNGRC; Drug Delivery Systems - methods; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent probe; Fundamental and applied biological sciences. Psychology; Humans; Microscopy, Fluorescence - methods; Molecular imaging; Tumor-homing peptide; Vertebrates: endocrinology; Molecular imaging; Aminopeptidase N (APN/CD13); CNGRC; Tumor-homing peptide; Fluorescent probe; Targeting; Peptides; Enzyme; Aminopeptidases; Biosynthesis; Peptidases; Imaging; Hydrolases; Tumor; Tumor cell; Membrane alanyl aminopeptidase
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2005.03.049

A family of fluorescein–peptide conjugates (CNP1–3) for aminopeptidase N (APN/CD13) targeting fluorescent probes were designed and synthesized. Among the three conjugates, CNP1 bearing tumor-homing cyclic peptide CNGRC, could selectively label APN/CD13 over-expressing on the surface of tumor cells of HT-1080, as identified by means of fluorescent microscopic cell imaging. CNP1 was shown to be a promising fluorescent probe applicable to tumor-targeting molecular imaging.