Radically Different Amyloid Conformations Dictate the Seeding Specificity of a Chimeric Sup35 Prion

• Published in: Journal of molecular biology Vol. 408; no. 1; pp. 1 - 8
• Main Authors: Foo, Catherine K., Ohhashi, Yumiko, Kelly, Mark J.S., Tanaka, Motomasa, Weissman, Jonathan S.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 22-04-2011
• Subjects: amides; Amino Acid Sequence; Amyloid; Amyloid - chemistry; beta -Amyloid; Candida albicans; Candida albicans - metabolism; Chimeras; chimerism; Fibers; Fungal Proteins - chemistry; Fungal Proteins - metabolism; hydrogen/deuterium exchange; Memory; Molecular Sequence Data; Peptide Termination Factors - chemistry; Peptide Termination Factors - metabolism; phenotype; Plasticity; Prion Diseases - transmission; Prion protein; prion species barrier; prion strain; prions; Prions - chemistry; Prions - metabolism; Probes; Protein Conformation; protein misfolding; Proteolysis; PSI; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - metabolism; Seeding; Seeds; Species Specificity; yeasts; SC; HX; DMSO; prion strain; protein misfolding; PSI; prion species barrier; CA; hydrogen/deuterium exchange
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/j.jmb.2011.02.025

A remarkable feature of prion biology is that the same prion protein can misfold into more than one infectious conformation, and these conformations in turn lead to distinct heritable prion strains with different phenotypes. The yeast prion [PSI+] is a powerful system for studying how changes in strain conformation affect cross-species transmission. We have previously established that a chimera of the Saccharomyces cerevisiae (SC) and Candida albicans (CA) Sup35 prion domains can cross the SC/CA species barrier in a strain-dependent manner. In vitro, the conversion of the monomeric chimera into the prion (amyloid) form can be seeded by either SC or CA Sup35 amyloid fibers, resulting in two strains: Chim[SC] and Chim[CA]. These strains have a “molecular memory” of their originating species in that Chim[SC] preferentially seeds the conversion of SC Sup35, and vice versa. To investigate how this species specificity is conformationally encoded, we used amide exchange and limited proteolysis to probe the structures of these two strains. We found that the amyloid cores of Chim[SC] and Chim[CA] are predominantly confined to the SC-derived and CA-derived residues, respectively. In addition, the chimera is able to propagate the Chim[CA] conformation even when the SC residues comprising the Chim[SC] core were deleted. Thus, the two strains have non-overlapping and modular amyloid cores that determine whether SC or CA residues are presented on the growing face of the prion seed. These observations establish how conformations determine the specificity of prion transmission and demonstrate a remarkable plasticity to amyloid misfolding.
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► A chimera of Sc and Ca Sup35 prion domains forms two different strain conformations. ► These strains preferentially seed polymerization of Sc or Ca Sup35, respectively. ► The amyloid cores of these strains are in non-overlapping regions of Chimera. ► The location of the amyloid core determines the seeding specificity of the strain.