Multifunctional Biomimetic Nanofibrous Scaffold Loaded with Asiaticoside for Rapid Diabetic Wound Healing

• Published in: Pharmaceutics Vol. 14; no. 2; p. 273
• Main Authors: Anand, Sneha, Rajinikanth, Paruvathanahalli Siddalingam, Arya, Dilip Kumar, Pandey, Prashant, Gupta, Ravi K, Sankhwar, Ruchi, Chidambaram, Kumarappan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24-01-2022; MDPI
• Subjects: asiaticoside; Biopolymers; crosslinking; Diabetes; diabetic wound healing; Drug delivery systems; electrospinning; extracellular matrix; Foot diseases; Laboratories; Morphology; nanofibers; Polyvinyl alcohol; Scanning electron microscopy; Tissue engineering; Ulcers; Wound healing; Mumbai India; India; asiaticoside; electrospinning; polyvinyl alcohol; sodium alginate; crosslinking; diabetic wound healing; extracellular matrix; nanofibers
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics14020273

Diabetes mellitus is a chronic disease with a high mortality rate and many complications. A non-healing diabetic foot ulcer (DFU) is one the most serious complications, leading to lower-extremity amputation in 15% of diabetic patients. Nanofibers are emerging as versatile wound dressing due to their unique wound healing properties, such as a high surface area to volume ratio, porosity, and ability to maintain a moist wound environment capable of delivering sustained drug release and oxygen supply to a wound. The present study was aimed to prepare and evaluate a polyvinyl alcohol (PVA)-sodium alginate (SA)-silk fibroin (SF)-based multifunctional nanofibrous scaffold loaded with asiaticoside (AT) in diabetic rats. The SEM findings showed that fibers' diameters ranged from 100-200 nm, and tensile strengths ranged from 12.41-16.80 MPa. The crosslinked nanofibers were sustained AT over an extended period. The MTT and scratch assay on HaCat cells confirmed low cytotoxicity and significant cell migration, respectively. Antimicrobial tests revealed an excellent anti-microbial efficacy against
and
bacteria. In-vivo study demonstrated better wound healing efficacy in diabetic rats. In addition, the histopathological studies showed its ability to restore the normal structure of the skin. The present study concluded that developed multifunctional nanofibers have a great potential for diabetic wound healing applications.