Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing Neuritin

• Published in: Antibodies (Basel) Vol. 12; no. 2; p. 28
• Main Authors: Papadogianni, Georgia, Ravens, Inga, Hassan, Ahmed, Flatley, Andrew, Feederle, Regina, Bernhardt, Günter, Georgiev, Hristo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07-04-2023; MDPI
• Subjects: Alzheimer's disease; Angiogenesis; B cells; blocking antibody; Cell growth; Cell migration; Cloning; Communication; Developmental plasticity; ELISA; Flow cytometry; Health aspects; Identification and classification; Immunization; Immunoregulation; Laboratories; Lymphatic system; Lymphocytes; Lymphocytes B; Lymphocytes T; Monoclonal antibodies; monoclonal antibody; neuritin; Neuropeptides; Neurotrophic factors; Neurotrophic functions; Peptides; Physiological aspects; Proteins; Structure; T cells; Testing; Wildlife conservation; Germany; United States > US; Berlin Germany; blocking antibody; neuritin; flow cytometry; monoclonal antibody; ELISA
• ISSN: 2073-4468; 2073-4468
• DOI: 10.3390/antib12020028

Neuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived peptides. Because neuritin is well conserved between species, these new monoclonal antibodies recognize the neuritin of a wide variety of species, including human. Moreover, they not only recognize specifically surface-bound neuritin expressed by murine follicular regulatory T cells but also the block binding of recombinant neuritin to germinal center B cells. This suggests that these newly generated tools will be of great use in studying neuritin expression and function.