Bacteria-Targeted Clindamycin Loaded Polymeric Nanoparticles: Effect of Surface Charge on Nanoparticle Adhesion to MRSA, Antibacterial Activity, and Wound Healing

• Published in: Pharmaceutics Vol. 11; no. 5; p. 236
• Main Authors: Hasan, Nurhasni, Cao, Jiafu, Lee, Juho, Hlaing, Shwe Phyu, Oshi, Murtada A, Naeem, Muhammad, Ki, Min-Hyo, Lee, Bok Luel, Jung, Yunjin, Yoo, Jin-Wook
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15-05-2019; MDPI
• Subjects: antibacterial; Antibiotics; Antimicrobial agents; Bacteria; Bacterial infections; clindamycin; Drug delivery systems; Morphology; MRSA-infected wound healing; Nanoparticles; Particle size; Scanning electron microscopy; Staphylococcus infections; surface charge; Wound healing; United States > US; Germany; surface charge; MRSA-infected wound healing; antibacterial; nanoparticles; clindamycin
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics11050236

Adhesion of nanoparticles (NPs) to the bacterial cell wall by modifying their physicochemical properties can improve the antibacterial activity of antibiotic. In this study, we prepared positively charged clindamycin-loaded poly (lactic-co-glycolic acid)-polyethylenimine (PLGA-PEI) nanoparticles (Cly/PPNPs) and negatively charged clindamycin-loaded PLGA NPs (Cly/PNPs) and investigated the effect of NP adhesion to bacteria on the treatment of methicillin-resistant
(MRSA)-infected wounds. The Cly/PPNPs and Cly/PNPs were characterized according to particle size, polydispersity index, surface charge, and drug loading. Both Cly/PPNPs and Cly/PNPs exhibited sustained drug release over 2 days. The Cly/PPNPs bind to the MRSA surface, thereby enhancing bactericidal efficacy against MRSA compared with the Cly/PNPs. Furthermore, compared with other groups, Cly/PPNPs significantly accelerated the healing and re-epithelialization of wounds in a mouse model of a MRSA-infected wounds. We also found that both NPs are harmless to healthy fibroblast cells. Therefore, our results suggest that the Cly/PPNPs developed in this study improve the efficacy of clindamycin for the treatment of MRSA-infected wounds.