Asparagine-linked carbohydrate of Penicillium notatum phospholipase B

Asparagine-linked carbohydrate of Penicillium notatum phospholipase B

The asparagine-linked sugar chains of phospholipase B from Penicillium notatum were released by hydrazinolysis. The carbohydrate components were re-N-acetylated and then reductively aminated with 2-aminopyridine. The pyridyl-aminated derivatives were analyzed by size-fractionation and reverse-phase...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) Vol. 116; no. 1; p. 204
Main Authors: Fujii, S, Unezaki, S, Okumura, T, Miura, R, Saito, K
Format: Journal Article
Language:English
Published: England 1994
Subjects:
Asparagine - chemistry
Carbohydrate Conformation
Carbohydrate Sequence
Lysophospholipase - chemistry
Molecular Sequence Data
Oligosaccharides - chemistry
Penicillium - enzymology
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Description
Summary:The asparagine-linked sugar chains of phospholipase B from Penicillium notatum were released by hydrazinolysis. The carbohydrate components were re-N-acetylated and then reductively aminated with 2-aminopyridine. The pyridyl-aminated derivatives were analyzed by size-fractionation and reverse-phase HPLC. The results indicated that the enzyme contains only one kind of oligosaccharide. The carbohydrate was purified by HPLC, and then subjected to NMR and MS analyses for determination of its structure. Based on the results, the structure was determined to be as follows. [sequence: see text]
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a124494