Common gene variants in RAD51, XRCC2 and XPD are not associated with clinical outcome in soft-tissue sarcoma patients

• Published in: Cancer epidemiology Vol. 37; no. 6; pp. 1003 - 1009
• Main Authors: Szkandera, Joanna, Absenger, Gudrun, Liegl-Atzwanger, Bernadette, Pichler, Martin, Stotz, Michael, Gerger, Stefan, Zacherl, Maximilian, Renner, Wilfried, Haijun, Miao, Leithner, Andreas, Gerger, Armin
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01-12-2013; Elsevier; Elsevier Limited
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Analysis. Health state; Biological and medical sciences; Cancer; Clinical outcomes; Combined Modality Therapy; DNA Repair; DNA, Neoplasm - genetics; DNA-Binding Proteins - genetics; Epidemiology; Female; Follow-Up Studies; Gene variant; General aspects; Genes; Hematology, Oncology and Palliative Medicine; Homologous recombination; Humans; Internal Medicine; Male; Medical sciences; Middle Aged; Mortality; Neoplasm Grading; Nucleotide excision repair; Polymerase Chain Reaction; Polymorphism, Genetic - genetics; Prognosis; Public health. Hygiene; Public health. Hygiene-occupational medicine; Rad51 Recombinase - genetics; Retrospective Studies; Sarcoma - genetics; Sarcoma - mortality; Sarcoma - therapy; Soft-tissue sarcoma; Survival Rate; Tumors; Xeroderma Pigmentosum Group D Protein - genetics; Young Adult; Nucleotide excision repair; Gene variant; Homologous recombination; Soft-tissue sarcoma; Human; Prognosis; Genetic variability; Soft tissue sarcoma; Genotype; Malignant tumor; Repair gene; Variant; Association; Evolution; Genetics; Cancer
• ISSN: 1877-7821; 1877-783X
• DOI: 10.1016/j.canep.2013.10.003

Abstract Background DNA repair mechanisms play a major role in cancer risk and progression. Germline variants in DNA repair genes may result in altered gene function and/or activity, thereby causing inter-individual differences in a patient's tumor recurrence capacity. In genes of the DNA repair pathway the gene variants RAD51 rs1801320 G > C, XRCC2 rs3218536 G > A and XPD rs13181 A > C have been previously related to genetic predisposition and prognosis of various cancer entities. In this study we investigated the association between these polymorphisms and time to recurrence (TTR) and overall survival (OS) in soft-tissue sarcoma (STS) patients after curative surgery. Methods Two hundred sixty STS patients were included in this retrospective study. Germline DNA was genotyped by 5′-exonuclease (TaqMan) technology. Kaplan Meier curves and multivariate Cox proportional models were calculated for TTR and OS. Results A statistically significant association was observed between tumor grade and adjuvant radiotherapy and TTR and between tumor grade and OS. No association was found between RAD51 rs1801320 G > C, XRCC2 rs3218536 G > A and XPD rs13181 A > C and TTR and OS in univariate and multivariate analysis. Conclusion Our results underline a prognostic effect of tumor grade and adjuvant radiotherapy in STS patients but indicate no association between RAD51 rs1801320 G > C, XRCC2 rs3218536 G > A and XPD rs13181 A > C and clinical outcome in STS patients after curative surgery.