The Polyphenol Chlorogenic Acid Attenuates UVB-mediated Oxidative Stress in Human HaCaT Keratinocytes

• Published in: Biomolecules & therapeutics Vol. 22; no. 2; pp. 136 - 142
• Main Authors: Cha, Ji Won, Piao, Mei Jing, Kim, Ki Cheon, Yao, Cheng Wen, Zheng, Jian, Kim, Seong Min, Hyun, Chang Lim, Ahn, Yong Seok, Hyun, Jin Won
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society of Applied Pharmacology 31-03-2014; 한국응용약물학회
• Subjects: Original; 약학; Oxidative stress; Chlorogenic acid; Human keratinocyte; Ultraviolet B; Apoptosis
• ISSN: 1976-9148; 2005-4483; 1976-9148; 2005-4483
• DOI: 10.4062/biomolther.2014.006

We investigated the protective effects of chlorogenic acid (CGA), a polyphenol compound, on oxidative damage induced by UVB exposure on human HaCaT cells. In a cell-free system, CGA scavenged 1,1-diphenyl-2-picrylhydrazyl radicals, superoxide anions, hydroxyl radicals, and intracellular reactive oxygen species (ROS) generated by hydrogen peroxide and ultraviolet B (UVB). Furthermore, CGA absorbed electromagnetic radiation in the UVB range (280-320 nm). UVB exposure resulted in damage to cellular DNA, as demonstrated in a comet assay; pre-treatment of cells with CGA prior to UVB irradiation prevented DNA damage and increased cell viability. Furthermore, CGA pre-treatment prevented or ameliorated apoptosis-related changes in UVB-exposed cells, including the formation of apoptotic bodies, disruption of mitochondrial membrane potential, and alterations in the levels of the apoptosis-related proteins Bcl-2, Bax, and caspase-3. Our findings suggest that CGA protects cells from oxidative stress induced by UVB radiation.