Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens

Cancer cell heterogeneity is well-documented. Therefore, techniques to monitor single cell heterogeneous responses to treatment are needed. We developed a highly translational and quantitative bioluminescence microscopy method to measure single cell androgen receptor (AR) activity modulation by anti...

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Bibliographic Details
Published in:	Scientific reports Vol. 6; no. 1; p. 33968
Main Authors:	Jain, Pallavi, Neveu, Bertrand, Velot, Lauriane, Wu, Lily, Fradet, Yves, Pouliot, Frédéric
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 28-09-2016 Nature Publishing Group
Subjects:	14 14/63 692/4028/67/2321 692/4028/67/2329 692/4028/67/589/466 Androgen receptors Androgens Antiandrogens Bioluminescence Cancer Heterogeneity Humanities and Social Sciences Microscopy multidisciplinary Science
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Summary:	Cancer cell heterogeneity is well-documented. Therefore, techniques to monitor single cell heterogeneous responses to treatment are needed. We developed a highly translational and quantitative bioluminescence microscopy method to measure single cell androgen receptor (AR) activity modulation by antiandrogens from fluid biopsies. We showed that this assay can detect heterogeneous cellular response to drug treatment and that the sum of single cell AR activity can mirror the response in the whole cell population. This method may thus be used to monitor heterogeneous dynamic treatment responses in cancer cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep33968