Testing of the mutagenicity and genotoxicity of metolcarb by using both Ames/ Salmonella and Allium test

• Published in: Chemosphere (Oxford) Vol. 80; no. 9; pp. 1056 - 1061
• Main Authors: Liman, Recep, Akyıl, Dilek, Eren, Yasin, Konuk, Muhsin
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-08-2010; Elsevier
• Subjects: Acaricide; Allium cepa; Anaphase; Animal, plant and microbial ecology; Applied ecology; Applied sciences; Biological and medical sciences; Chromosome aberration; Ecotoxicology, biological effects of pollution; Environmental Exposure; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General aspects; Insecticide; Mitosis; Mitotic index; Mutagenicity Tests; Mutagens - toxicity; Onions - drug effects; Onions - growth & development; Phenylcarbamates - toxicity; Plant Roots - cytology; Plant Roots - drug effects; Plant Roots - genetics; Pollution; Polyploidy; Salmonella typhimurium; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics; Telophase; Time Factors; Water Pollutants, Chemical - toxicity; Mitotic index; Acaricide; Insecticide; Chromosome aberration; Chromosomal aberration; Salmonella; Monocotyledones; Ecotoxicology; Toxicity; Pesticides; Genotoxicity; Allium; Liliaceae; Angiospermae; Bacteria; Spermatophyta; Organic compounds; Enterobacteriaceae
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2010.05.011

Mutagenic and genotoxic effects of metolcarb were investigated by both bacterial reverse mutation assay in
Salmonella typhimurium TA97, TA98, TA100 and TA102 strains with or without metabolic activation system (S9) and
Allium cepa root meristematic cells, respectively. Metolcarb was dissolved in DMSO in Ames/
Salmonella test system. 0.1, 1 and 10
μg/plate doses of metolcarb were found to be mutagenic
S. typhimurium TA98 without S9. In
Allium root growth inhibition test, EC
50 value was determined 200
ppm and 0.5xEC
50, EC
50 and 2xEC
50 concentrations of metolcarb were introduced to onion tuber roots and distilled water used as a negative control. Mitotic index (MI), increased in all concentrations compared to control at each exposure time. While disturbed anaphase–telophase, chromosome laggards, stickiness and bridges were observed in anaphase–telophase cells, pro-metaphase, C-mitosis, polyploidy, binuclear cells and disturbed nucleus were observed in other cells. The results were also analyzed statistically by using SPSS for Windows, Mann–Whitney test and Duncan’s multiple range tests were performed respectively.