Combination of a TLR4 ligand and anaphylatoxin C5a for the induction of antigen-specific cytotoxic T cell responses

• Published in: Vaccine Vol. 30; no. 18; pp. 2848 - 2858
• Main Authors: Rudilla, Francesc, Fayolle, Catherine, Casares, Noelia, Durantez, Maika, Arribillaga, Laura, Lozano, Teresa, Villanueva, Lorea, Pio, Ruben, Sarobe, Pablo, Leclerc, Claude, Prieto, Jesús, Lasarte, Juan José
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 16-04-2012; Elsevier Limited
• Subjects: adaptive immunity; Adjuvant; Allergy and Immunology; Anaphylatoxin C5a; Anaphylatoxins; Anaphylatoxins - genetics; Anaphylatoxins - immunology; Animals; Antigen-presenting cells; Antigens; Antiviral agents; C5a; Cancer Vaccines - administration & dosage; Cancer Vaccines - immunology; CCL17 protein; Cell activation; cell movement; Chemokines; complement; Complement C5a - genetics; Complement C5a - immunology; complement component C5a; CXCL12 protein; Cytokines; Cytokines - secretion; Cytotoxicity; Dendritic cell; Dendritic cells; DNA; Ectodysplasins - genetics; Ectodysplasins - immunology; EDA; Epitopes; Epitopes, T-Lymphocyte - genetics; Epitopes, T-Lymphocyte - immunology; Female; Fibronectin; fibronectins; Fusion protein; immune response; Immune system; Immunization; Immunogenicity; Inflammation; Interleukin 10; Leukocyte migration; Lymphocytes T; Mice; Mice, Inbred C57BL; Myeloid cells; Natural killer cells; Neoplasms - immunology; Neoplasms - prevention & control; Ovalbumin; Plasmids; recombinant fusion proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; T-lymphocytes; T-Lymphocytes, Cytotoxic - immunology; TLR4 protein; Toll-Like Receptor 4 - agonists; Toll-like receptors; Tumors; Vaccine; Vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - immunology; bone marrow derived DC; EDA; extra domain A from fibronectin; Poly(I:C); Vaccine; polyinosine–polycytidylic acid; C5a; APC; BMDC; antigen presenting cell; Adjuvant; Fusion protein; dendritic cell; DC; Dendritic cell
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2012.02.052

Highlights ► Anaphylatoxin C5a and the extra domain A (EDA) from fibronectin induce DC maturation. ► Fusion of C5a and EDA to an antigen improve the immunogenicity of the antigen in vivo. ► EDA–C5a–antigen fusion proteins can be useful as anti-tumor/anti-viral vaccines.