Microbial communities in bulk fluids and biofilms of an oil facility have similar composition but different structure

• Published in: Environmental microbiology Vol. 13; no. 4; pp. 1078 - 1090
• Main Authors: Stevenson, Bradley S., Drilling, Heather S., Lawson, Paul A., Duncan, Kathleen E., Parisi, Victoria A., Suflita, Joseph M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-04-2011
• Subjects: Archaea; Archaea - classification; Archaea - genetics; Archaea - growth & development; Bacteria - classification; Bacteria - genetics; Bacteria - growth & development; Biofilms; Corrosion; DNA, Archaeal - genetics; DNA, Bacterial - genetics; Firmicutes; Gene Library; Petroleum - microbiology; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Thermoanaerobacter
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/j.1462-2920.2010.02413.x

Summary
The oil–water–gas environments of oil production facilities harbour abundant and diverse microbial communities that can participate in deleterious processes such as biocorrosion. Several molecular methods, including pyrosequencing of 16S rRNA libraries, were used to characterize the microbial communities from an oil production facility on the Alaskan North Slope. The communities in produced water and a sample from a ‘pig envelope’ were compared in order to identify specific populations or communities associated with biocorrosion. The ‘pigs’ are used for physical mitigation of pipeline corrosion and fouling and the samples are enriched in surface‐associated solids (i.e. paraffins, minerals and biofilm) and coincidentally, microorganisms (over 105‐fold). Throughout the oil production facility, bacteria were more abundant (10‐ to 150‐fold) than archaea, with thermophilic members of the phyla Firmicutes (Thermoanaerobacter and Thermacetogenium) and Synergistes (Thermovirga) dominating the community. However, the structure (relative abundances of taxa) of the microbial community in the pig envelope was distinct due to the increased relative abundances of the genera Thermacetogenium and Thermovirga. The data presented here suggest that bulk fluid is representative of the biofilm communities associated with biocorrosion but that certain populations are more abundant in biofilms, which should be the focus of monitoring and mitigation strategies.