The superior regenerative potential of muscle-derived stem cells for articular cartilage repair is attributed to high cell survival and chondrogenic potential

• Published in: Molecular therapy. Methods & clinical development Vol. 3; no. C; p. 16065
• Main Authors: Li, Hongshuai, Lu, Aiping, Tang, Ying, Beckman, Sarah, Nakayama, Naoki, Poddar, Minakshi, Hogan, MaCalus V, Huard, Johnny
• Format: Journal Article
• Language: English
• Published: United States Elsevier Limited 01-01-2016; Nature Publishing Group; Elsevier
• Subjects: Cartilage (articular); Cell survival; Collagen; Defects; Deoxyribonucleic acid; DNA; Fibroblasts; Gene therapy; Growth factors; Joint surgery; Musculoskeletal system; Myoblasts; Oxidative stress; Population; Skeletal muscle; Statistical analysis; Stem cell transplantation; Stem cells; Variance analysis; Japan
• ISSN: 2329-0501; 2329-0501
• DOI: 10.1038/mtm.2016.65

Three populations of muscle-derived cells (PP1, PP3, and PP6) were isolated from mouse skeletal muscle using modified preplate technique and retrovirally transduced with BMP4/GFP.  
, the PP1 cells (fibroblasts) proliferated significantly slower than the PP3 (myoblasts) and PP6 cells (muscle-derived stem cells); the PP1 and PP6 cells showed a superior rate of survival compared with PP3 cells under oxidative stress; and the PP6 cells showed significantly superior chondrogenic capabilities than PP1 and PP3 cells.
, the PP6 cells promoted superior cartilage regeneration compared with the other muscle-derived cell populations. The cartilage defects in the PP6 group had significantly higher histological scores than those of the other muscle-derived cell groups, and GFP detection revealed that the transplanted PP6 cells showed superior
cell survival and chondrogenic capabilities compared with the PP1 and PP3 cells. PP6 cells (muscle-derived stem cells) are superior to other primary muscle-derived cells for use as a cellular vehicle for BMP4-based
gene therapy to heal full-thickness osteo-chondral defects. The superiority of the PP6/muscle-derived stem cells appears to be attributable to a combination of increased rate of
survival and superior chondrogenic differentiation capacity.