A scaling law for membrane permeabilization with nanopulses

• Published in: IEEE transactions on dielectrics and electrical insulation Vol. 16; no. 5; pp. 1224 - 1235
• Main Authors: Schoenbach, K., Joshi, R., Beebe, S., Baum, C.
• Format: Journal Article
• Language: English
• Published: New York IEEE 01-10-2009; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Bioelectric phenomena; Biological cells; Biomembranes; Cells (biology); Electric charge; Electric fields; electropermeabilization; electroporation; Law; Membrane effects; Membranes; Nanobioscience; Nanomaterials; nanoporation; Nanoporous materials; nanosecond electric fields; Nanostructure; Permeability; Plasma transport processes; Plasma waves; random rotation; Roots; Similarity; Studies; USA Councils
• ISSN: 1070-9878; 1558-4135
• DOI: 10.1109/TDEI.2009.5293932

Experimental studies of plasma membrane permeabilization, caused by single, intense, submicrosecond square wave pulses, indicate that the product of electric field amplitude and pulse duration (the electrical impulse) can be considered a similarity or scaling factor. A model based on the hypothesis that the intensity of membrane permeabilization effects is linearly dependent on the electric charge transferred through the permeabilized membrane, provides results, which are consistent with the empirical observations. For multiple pulses, bioelectric effects caused by ultrashort pulses were found to scale with the square root of the pulse number. This square root dependence on the pulse number points to a statistical motion of cells between pulses with respect to the applied electric field, and can be explained using an extension of the random walk statistical results to random rotations. Besides membrane permeabilization, the scaling law has also been shown to hold for secondary bioelectric effects, which are caused by permeability changes in the plasma membrane or subcellular membranes.