Fabrication of an Impedimetric Immunosensor for Screening and Determination of Vincristine in Biological Samples

• Published in: Journal of analytical chemistry (New York, N.Y.) Vol. 75; no. 8; pp. 1094 - 1101
• Main Authors: Haidar, Saify Nabiabad, Amini, Massoumeh
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-08-2020; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Biological products; Biological properties; Biosensors; Chemistry; Chemistry and Materials Science; Detectors; Electric properties; Electrochemical analysis; Electrochemical impedance spectroscopy; Glassy carbon; Health aspects; Herbal medicine; Immunosensors; Medicinal plants; Medicine, Botanic; Medicine, Herbal; Monoclonal antibodies; Screening; Terpenes; Tubes; Video equipment industry; Video tape recorders and recording; Vincristine; impedance spectroscopy; MWCNTs; antibody; biosensor; vincristine
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934820080092

Terpenoids of medicinal plants are one of the most important sources of therapeutic drugs. Among them, Vincristine (
VCR
) is prescribed for a wide variety of cancer types. Therefore, development of sensitive methods for VCR determination is urgently required. Here, a label-free and highly sensitive impedimetric biosensor was fabricated for electrochemical detection of VCR. First, multi-wall carbon nano tubes (
MWCNTs
) were dropped on the surface of a glassy carbon electrode. Then, the VCR monoclonal antibody (
Ab
) was covalently immobilized on the MWCNTs via carbodiimide coupling reaction using N-(3-dimethylaminopropyl)-N'-ethyl carbodiimide and N-hydroxy succinimide. The step-by-step modification process was characterized by electrochemical impedance spectroscopy and cyclic voltammetry in presence of a redox probe [Fe(CN)
6
]
3–/4–
. The VCR concentration was measured through increasing impedance values in the corresponding specific binding of VCR to the Ab. The increased electron-transfer resistance (
R
et
) values were proportional to the concentration of VCR and the biosensor detected concentrations varied in the range of 0.2–50 nM with a detection limit of 8 × 10
–2
nM (S/N = 3). Practical analytical utility of the proposed method was demonstrated by VCR determination in medicinal plant extracts and human serum samples with satisfactory recoveries. The proposed biosensor may be a promising alternative for screening of medicinal plants to produce VCR and for the determination of drug concentration in cancerous potions.