Wound healing potential of antibacterial microneedles loaded with green tea extracts

• Published in: Materials Science & Engineering C Vol. 42; pp. 757 - 762
• Main Authors: Park, So Young, Lee, Hyun Uk, Lee, Young-Chul, Kim, Gun Hwa, Park, Edmond Changkyun, Han, Seung Hyun, Lee, Jeong Gyu, Choi, Saehae, Heo, Nam Su, Kim, Dong Lak, Huh, Yun Suk, Lee, Jouhahn
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-09-2014
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antiinfectives and antibacterials; Bacillus subtilis; Bacteria; Bacteria - drug effects; Bacterial Infections; Camellia sinensis - chemistry; CHO Cells; Cricetinae; Cricetulus; Disease Models, Animal; Drug Delivery Systems; Escherichia coli; Fourier transforms; Green tea; Green tea extract; H alpha line; HEK293 Cells; Humans; Hyaluronic acid; Hyaluronic Acid - chemistry; Hydroxyapatite; Male; Microneedles; Microtechnology - instrumentation; Needles; Plant Extracts - chemistry; Plant Extracts - pharmacology; Pseudomonas putida; Rats, Sprague-Dawley; Salmonella typhimurium; Staphylococcus aureus; Transdermal drug delivery; Transdermal Patch; Wound healing; Wound Healing - drug effects; Transdermal drug delivery; Green tea extract; Antibacterial activity; Microneedles; Wound healing; Hyaluronic acid
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2014.06.021

This study evaluates the utility of an antibacterial microneedle composed of green tea (GT) extract and hyaluronic acid (HA), for the efficient delivery of GT. These microneedles have the potential to be a patient-friendly method for the conventional sustained release of drugs. In this study, a fabrication method using a mold-based technique to produce GT/HA microneedles with a maximum area of ~50mm2 with antibacterial properties was used to manufacture transdermal drug delivery systems. Fourier transform infrared (FTIR) spectrometry was carried out to observe the potential modifications in the microneedles, when incorporated with GT. The degradation rate of GT in GT/HA microneedles was controlled simply by adjusting the HA composition. The effects of different ratios of GT in the HA microneedles were determined by measuring the release properties. In HA microneedles loaded with 70% GT (GT70), a continuous higher release rate was sustained for 72h. The in vitro cytotoxicity assays demonstrated that GT/HA microneedles were not generally cytotoxic to Chinese hamster ovary cells (CHO-K1), human embryonic kidney cells (293T), and mouse muscle cells (C2C12), which were treated for 12 and 24h. Antimicrobial activity of the GT/HA microneedles was demonstrated by ~95% growth reduction of gram negative [Escherichia coli (E. coli), Pseudomonas putida (P. putida), and Salmonella typhimurium (S. typhimurium)] and gram positive bacteria [Staphylococcus aureus (S. Aureus) and Bacillus subtilis (B. subtilis)], with GT70. Furthermore, GT/HA microneedles reduced bacterial growth of infected wound sites in the skin and improved wound healing process of skin in rat model.
Green Tea/hyaluronic acid microneedle suppresses bacterial growth in the wound skin sites and improves wound healing. [Display omitted]
•Antibacterial microneedles composed of green tea extract and hyaluronic acid•A maximum area of ~50mm2 for transdermal drug delivery systems•No in vitro cytotoxic effect in mammalian cells•~95% growth reduction of gram negative and positive bacteria•Improvement of wound healing process in rat skin model