IL-1Rrp Is a Novel Receptor-like Molecule Similar to the Type I Interleukin-1 Receptor and Its Homologues T1/ST2 and IL-1R AcP (∗)

• Published in: The Journal of biological chemistry Vol. 271; no. 8; pp. 3967 - 3970
• Main Authors: Parnet, Patricia, Garka, Kirsten E., Bonnert, Timothy P., Dower, Steven K., Sims, John E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23-02-1996; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; Animals; Cell Line; Cell Membrane - metabolism; Chlorocebus aethiops; Cloning, Molecular; Cytoplasm - metabolism; DNA Primers; Humans; Interleukin-18 Receptor alpha Subunit; Interleukin-8 - biosynthesis; Interleukin-8 - genetics; Mice; Molecular Sequence Data; NF-kappa B - metabolism; Polymerase Chain Reaction; Promoter Regions, Genetic; Protein Biosynthesis; Proteins - chemistry; Receptors, Interleukin-1 - biosynthesis; Receptors, Interleukin-1 - chemistry; Receptors, Interleukin-18; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - chemistry; Sequence Homology, Amino Acid; Transfection
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.271.8.3967

A novel member of the interleukin-1 receptor family has been cloned by polymerase chain reaction using degenerate oligonucleotide primers derived from regions of sequence conservation, using as template a yeast artificial chromosome known to contain both interleukin-1 (IL-1) receptors and T1/ST2. The new receptor, called IL-1 receptor-related protein or IL-1Rrp, fails to bind any of the known IL-1 ligands. A chimeric receptor, in which the IL-1Rrp cytoplasmic domain is fused to the extracellular and transmembrane regions of the IL-1 receptor, responds to IL-1 following transfection into COS cells by activation of NFκB and induction of IL-8 promoter function.