Activated platelets induce superoxide anion release by monocytes and neutrophils through P-selectin (CD62)

• Published in: The Journal of immunology (1950) Vol. 151; no. 6; pp. 3267 - 3273
• Main Authors: Nagata, K, Tsuji, T, Todoroki, N, Katagiri, Y, Tanoue, K, Yamazaki, H, Hanai, N, Irimura, T
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15-09-1993; American Association of Immunologists
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Base Sequence; Biological and medical sciences; Blood Platelets - physiology; Cell Adhesion Molecules - physiology; Cell interactions; Cell Membrane - physiology; Fixatives; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glutaral - pharmacology; Humans; Immunobiology; In Vitro Techniques; Lewis X Antigen - physiology; Molecular Sequence Data; Monocytes - metabolism; Neutrophils - metabolism; Oligodeoxyribonucleotides - chemistry; P-Selectin; Platelet Activation; Platelet Membrane Glycoproteins - physiology; Recombinant Fusion Proteins - metabolism; Respiratory Burst; Superoxides - metabolism; Human; Cell culture; Hyperoxides; Platelet; Monocyte; Cell adhesion molecule; Stimulation; Activation; Respiratory burst; Neutrophil; Adhesion
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.151.6.3267

Activated platelets expressing P-selectin in their surfaces are known to adhere to monocytes and neutrophils. We examined the possibility that the leukocytes are functionally modified by their adhesion to activated platelets. We used human peripheral blood monocytes and neutrophils and measured superoxide anion generation by these cells cultured with platelets. The levels of superoxide anion production were found to be markedly elevated when thrombin-activated platelets were used. The extent of this enhancement was much smaller when leukocytes were cultured with resting platelets than activated platelets. The increase depended on incubation time and platelet concentration. The membranes prepared from activated platelets also induced superoxide anion production, but the culture supernatant of activated platelets did not. The enhanced superoxide anion production was inhibited by anti-P-selectin antibody, anti-sialyl-Lewis X antibody, or a soluble recombinant P-selectin fusion protein. These results indicate that the adhesion of activated platelets to the leukocytes through P-selectin was a crucial step for the activation of leukocyte function, and support the idea that activated platelets are actively involved in inflammation processes.