An atypical component of RNA-directed DNA methylation machinery has both DNA methylation-dependent and -independent roles in locus-specific transcriptional gene silencing

RNA-directed DNA methylation （RdDM） is an important de novo DNA methylation pathway in plants. RdDM mediates the transcriptional silencing of many endogenous genomic loci, most of which are transposon related. A for- ward genetics screen identified DTF1 （DNA-binding transcription factor 1） as a new...

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Bibliographic Details
Published in:	Cell research Vol. 21; no. 12; pp. 1691 - 1700
Main Authors:	Liu, Jun, Bai, Ge, Zhang, Cuijun, Chen, Wei, Zhou, Jinxing, Zhang, Suwei, Chen, Qing, Deng, Xin, He, Xin-Jian, Zhu, Jian-Kang
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01-12-2011 Nature Publishing Group
Subjects:	631/337/176/1988 631/337/176/2016 631/449 631/45/612/822 Accumulation Arabidopsis Arabidopsis - metabolism Arabidopsis Proteins - antagonists & inhibitors Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Biomedical and Life Sciences Cell Biology Deoxyribonucleic acid DNA DNA Methylation DNA甲基化 DNA结合 epigenetics Gene Silencing Genetic Loci Genetic screening Genetics genomics Life Sciences Mutation Original original-article RNA Interference RNA, Small Interfering - metabolism siRNA siRNAs Transcription factors Transcription, Genetic Transposons 定位点机械组成部分转录后基因沉默非典型 DNA methylation siRNA DTF1 transcriptional gene silencing RdDM
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Summary:	RNA-directed DNA methylation （RdDM） is an important de novo DNA methylation pathway in plants. RdDM mediates the transcriptional silencing of many endogenous genomic loci, most of which are transposon related. A for- ward genetics screen identified DTF1 （DNA-binding transcription factor 1） as a new component for RdDM in Arabi- dopsis. Loss-of-function mutations in DTF1 release the transcriptional silencing of RdDM target loci and reduce the accumulation of 24-nt small interfering RNAs （siRNAs） from some of the targets. Interestingly, in the dtfl mutant plants, the release of transcriptional gene silencing at solo-LTR is accompanied by decreased siRNA accumulation but not by reduced DNA methylation. These results suggest that DTF1 is an atypical component of RdDM and has both DNA methylation-dependent and -independent roles in transcriptional gene silencing. We suggest that besides DNA methylation, siRNAs may cause some other uncharacterized epigenetic modifications that lead to transcription- al gene silencing.
Bibliography:	RNA-directed DNA methylation （RdDM） is an important de novo DNA methylation pathway in plants. RdDM mediates the transcriptional silencing of many endogenous genomic loci, most of which are transposon related. A for- ward genetics screen identified DTF1 （DNA-binding transcription factor 1） as a new component for RdDM in Arabi- dopsis. Loss-of-function mutations in DTF1 release the transcriptional silencing of RdDM target loci and reduce the accumulation of 24-nt small interfering RNAs （siRNAs） from some of the targets. Interestingly, in the dtfl mutant plants, the release of transcriptional gene silencing at solo-LTR is accompanied by decreased siRNA accumulation but not by reduced DNA methylation. These results suggest that DTF1 is an atypical component of RdDM and has both DNA methylation-dependent and -independent roles in transcriptional gene silencing. We suggest that besides DNA methylation, siRNAs may cause some other uncharacterized epigenetic modifications that lead to transcription- al gene silencing. DTF 1; siRNA; DNA methylation; transcriptional gene silencing; RdDM 31-1568/Q ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 These three authors contributed equally to this work.
ISSN:	1001-0602 1748-7838
DOI:	10.1038/cr.2011.173