Folding of cytosine-based nucleolipid monolayer by guanine recognition at the air-water interface

• Published in: Journal of colloid and interface science Vol. 537; pp. 694 - 703
• Main Authors: G. Argudo, Pablo, Muñoz, Eulogia, Giner-Casares, Juan José, Martín-Romero, María Teresa, Camacho, Luis
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-03-2019
• Subjects: Air; Complementary base pairing; Computer simulations; Cytosine - chemistry; Cytosine-based nucleolipid; Guanine - chemistry; Langmuir monolayer; Lipids - chemistry; Molecular Dynamics Simulation; Molecular Structure; Particle Size; Pressure; Surface Properties; Water - chemistry; Cytosine-based nucleolipid; Complementary base pairing; Langmuir monolayer; Computer simulations
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2018.11.036

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Monolayers of a cytosine-based nucleolipid (1,2-dipalmitoyl-sn-glycero-3-(cytidine diphosphate) (ammonium salt), CDP-DG) at basic subphase have been prepared at the air-water interface both in absence and presence of guanine. The formation of the complementary base pairing is demonstrated by combining surface experimental techniques, i.e., surface pressure (π)–area (A), Brewster angle microscopy (BAM), infrared spectroscopy (PM-IRRAS) and computer simulations. A folding of the cytosine-based nucleolipid molecules forming monolayer at the air-water interface occurs during the guanine recognition as absorbate host and is kept during several compression-expansion processes under set experimental conditions. The specificity between nitrogenous bases has been also registered. Finally, mixed monolayers of CDP-DG and a phospholipid (1,2-dimyristoyl-sn-glycero-3-phosphate (sodium salt), DMPA) has been studied and a molecular segregation of the DMPA molecules has been inferred by the additivity rule.