Human Platelet Lysate as a Promising Growth-Stimulating Additive for Culturing of Stem Cells and other Cell Types

• Published in: Bulletin of experimental biology and medicine Vol. 156; no. 1; pp. 146 - 151
• Main Authors: Shanskii, Ya. D., Sergeeva, N. S., Sviridova, I. K., Kirakozov, M. S., Kirsanova, V. A., Akhmedova, S. A., Antokhin, A. I., Chissov, V. I.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-11-2013; Springer; Springer Nature B.V
• Subjects: Adult; Animals; Biomedical and Life Sciences; Biomedicine; Blood Platelets - chemistry; Cattle; Cell Biology; Cell Culture Techniques; Cell Extracts - chemistry; Cell Proliferation; Cholesterol; Culture Media - chemistry; Female; Growth; Humans; Insulin-Like Growth Factor I - chemistry; Internal Medicine; Laboratory Medicine; Male; Mesenchymal Stem Cells - physiology; Pathology; Platelet-Derived Growth Factor - chemistry; Transforming Growth Factor beta - chemistry; Vascular Endothelial Growth Factor A - chemistry; fetal calf serum; cell culture; human platelet lysate; multipotent mesenchymal stromal cells
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-013-2298-7

We compared the composition and biological activity of fetal calf serum and platelet lysate from donor platelet concentrate. In platelet lysate, the concentrations of alkaline phosphatase, lactate dehydrogenase, creatinine, and mineral metabolism parameters were lower, while parameters of lipid and protein metabolism were higher than in fetal calf serum. The concentrations of growth factors (platelet-derived (AA, AB, BB), vascular endothelial, insulin-like, and transforming growth factor β) in platelet lysate 1.7-148.7-fold surpassed the corresponding parameters in fetal calf serum. After replacement of fetal calf serum with platelet lysate in the culture medium (0, 25, 50, 75, and 100%), the count of multipotent mesenchymal stromal cells on day 7 (in comparison with day 1) increased by 154.8, 206.6, 228.2, 367.7, and 396.5%, respectively. Thus, platelet lysate can be an adequate non-xenogenic alternative for fetal calf serum.