Sequence and organization of barley yellow dwarf virus genomic RNA

• Published in: Nucleic acids research Vol. 16; no. 13; pp. 6097 - 6111
• Main Authors: MILLER, W. A, WATERHOUSE, P. M, GERLACH, W. L
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 11-07-1988
• Subjects: Amino Acid Sequence; Barley yellow dwarf virus; Base Sequence; Biological and medical sciences; Carnation mottle virus; DNA - analysis; Fundamental and applied biological sciences. Psychology; Genetics; Microbiology; Molecular Sequence Data; Nucleic Acid Conformation; Plant Viruses - genetics; RNA, Viral - analysis; Virology; Genetic mapping; Genome organization; Translation; Luteovirus; RNA; Nucleotide sequence; Enzyme; Gene expression; Secondary structure; Open reading frame; Virus; Messenger RNA; Gene; RNA polymerase RNA-dependent; Coat protein; Barley yellow dwarf virus; Molecular cloning
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/16.13.6097

The nucleotide sequence of the genomic RNA of barley yellow dwarf virus, PAV serotype was determined, except for the 5'-terminal base, and its genome organization deduced. The 5,677 nucleotide genome contains five large open reading frames (ORFs). The genes for the coat protein (1) and the putative viral RNA-dependent RNA polymerase were identified. The latter shows a striking degree of similarity to that of carnation mottle virus (CarMV). By comparison with corona- and retrovirus RNAs, it is proposed that a translational frameshift is involved in expression of the polymerase. An ORF encoding an Mr 49,797 protein (50K ORF) may be translated by in-frame readthrough of the coat protein stop codon. The coat protein, an overlapping 17K ORF, and a 3'6.7K ORF are likely to be expressed via subgenomic mRNAs.