Total and Differential Cell Counts as a Tool to Identify Intramammary Infections in Cows after Calving

• Published in: Animals (Basel) Vol. 11; no. 3; p. 727
• Main Authors: Zecconi, Alfonso, Meroni, Gabriele, Sora, Valerio, Mattina, Roberto, Cipolla, Micaela, Zanini, Lucio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07-03-2021; MDPI
• Subjects: Animal health; Antiinfectives and antibacterials; Bacteria; Cow's milk; Dairy cattle; Diagnosis; differential cell count; Health risks; Identification methods; Infections; Laboratories; Lactation; Markers; Mastitis; Milk; Pathogens; periparturient period; Software; Somatic cells; total cell count; Udder; United States > US; diagnosis; differential cell count; mastitis; total cell count; periparturient period
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani11030727

Milk differential somatic cells count (DSCC), made possible under field conditions by the recent availability of a high-throughput milk analyzer may represent an improvement in mastitis diagnosis. While an increasing number of studies reports data on DSCC on individual cow samples, very few concerns DSCC from quarter milk samples. This paper reports for the first time the results of a retrospective study aiming to assess the performance of total (SCC), DSCC, and a novel calculated marker (PLCC) measured on quarter milk samples as a method to identify cows at risk for intramammary infection (IMI) in the first 30 days after calving. Overall, 14,586 valid quarter milk samples (3658 cows) taken in the first 30 days of lactation were considered. Quarters with major pathogens (MP) IMI, as expected, showed significantly higher means for SCC, DSCC, and PLCC. The accuracy, sensitivity, and specificity of the diagnosis based on different cut-offs calculated by ROC analysis are relatively close among DSCC, PLCC, and SCC (up to cut-off of 200,000 cells/mL). However, decision-tree analysis which includes the costs of analysis, but also the costs of the actions taken after test results showed as PLCC has the lowest cost among the three markers, and PLCC and SCC are cost effective when MP prevalence is higher than 6-10%. This diagnostic approach is of high interest particularly when selective dry cow therapy is applied to improve animal health at the herd level.