Investigation of relationship between IL-6 gene variants and hypertension in Turkish population

• Published in: Cytotechnology (Dordrecht) Vol. 67; no. 6; pp. 947 - 954
• Main Authors: Karaman, Esin, Urhan Kucuk, Meral, Bayramoglu, Aysegul, Uzun Göçmen, Semire, Ercan, Süleyman, Guler, Halil Ibrahim, Kucukkaya, Yunus, Erden, Sema
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-12-2015; Springer Nature B.V
• Subjects: Biochemistry; Biomedicine; Biotechnology; Blood pressure; Body mass index; C gene; Cardiovascular disease; Chemistry; Chemistry and Materials Science; Cytokines; Gender; Gene polymorphism; High density lipoprotein; Hypertension; Inflammation; Interleukin 6; Myocardial infarction; Original Research; Plasma; Plasma levels; Polymorphism; Population genetics; Renal failure; Restriction fragment length polymorphism; Statistical analysis; Germany; Hypertension; CRP; IL-6 gene polymorphism; 174 G/C, −572 G/C gene variants
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1007/s10616-014-9732-1

Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a polymorphic gene which −174 G/C is a common and −572 G/C is a rare polymorphisms identified in promoter region. Publications on IL-6 gene polymorphisms raised the question whether this gene polymorphisms lead to susceptibility to HT or not. To investigate the effects of IL-6 gene −174 G/C (rs 1800795) and −572 G/C (rs1800796) polymorphisms on plasma IL-6 and CRP levels and their associations with hypertension disease in Turkish population we analyzed −174 G/C and −572 G/C polymorphisms and plasma IL-6 and CRP levels in 111 healthy controls and 108 hypertension patients from Adıyaman, Turkey. We determined the genotypes using polymerase chain reaction-restriction fragment length polymorphism and analyzed plasma levels of IL-6 by ELISA and CRP by automated standard biochemical methods. We have found no statistically significant differences between IL-6 gene −174 G/C and −572 G/C genotypes and allelic frequencies and IL-6 and CRP plasma levels and HT (
p
 > 0.05). No CC genotype was found in control subjects for −572 G/C polymorphism. In conclusion, we found relation to −174 G/C and −572 G/C gene variants between neither IL-6 and CRP levels nor hypertension. The −572 G allele and GG genotype are predominant in Turkish population in Adıyaman, Turkey whereas the CC genotype is very rare.