Methiocarb-induced oxidative damage following subacute exposure and the protective effects of vitamin E and taurine in rats

• Published in: Food and chemical toxicology Vol. 47; no. 7; pp. 1676 - 1684
• Main Authors: Ozden, Sibel, Catalgol, Betul, Gezginci-Oktayoglu, Selda, Arda-Pirincci, Pelin, Bolkent, Sehnaz, Alpertunga, Buket
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-07-2009; Elsevier
• Subjects: animal models; Animals; antioxidant activity; antioxidants; Antioxidants - metabolism; Antioxidants - pharmacology; Biological and medical sciences; biomarkers; Chemical and Drug Induced Liver Injury - enzymology; Chemical and Drug Induced Liver Injury - pathology; Chemical and Drug Induced Liver Injury - prevention & control; enzyme activity; glutathione; Glutathione - metabolism; hepatotoxicity; histopathology; inhibitors; Insecticides - antagonists & inhibitors; Insecticides - toxicity; Kidney Diseases - chemically induced; Kidney Diseases - pathology; Kidney Diseases - prevention & control; lipid peroxidation; Lipid Peroxidation - drug effects; Liver and kidney injury; Male; Medical sciences; Methiocarb; Methiocarb - antagonists & inhibitors; Methiocarb - toxicity; nephrotoxicity; Oxidative damage; oxidative stress; Oxidative Stress - drug effects; pretreatment; Rats; Rats, Wistar; risk assessment; Taurine; Taurine - pharmacology; toxicity testing; Toxicology; toxigenesis; Vitamin E; Vitamin E - pharmacology; NADPH; Masson; Taurine; C; MDA; SD; Liver and kidney injury; T; vit E + MC; Vitamin E; MC; T + MC; GSH; HPLC; GSSG; NBT; GSH-Px; i.g; DTNB; Oxidative damage; SOD; i.p; TBARS; FMOs; 0.9% NaCl; vit E; CAT; Methiocarb; Oxidative stress; Rat; Digestive system; Toxicity; Liver; Rodentia; Protective factor; Exposure; Kidney; Vertebrata; Mammalia; Urinary system; Aminoacid; Animal; Lesion; Subacute
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2009.04.018

Methiocarb, is used worldwide in agriculture and health programs. Besides its advantages in the agriculture, it causes several toxic effects. In this study, we aimed to investigate subacute effects of methiocarb on lipid peroxidation, reduced glutathione (GSH), antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rd) and histopathological changes in rat tissues. Moreover, we examined the possible protective effects of vitamin E and taurine on methiocarb-induced oxidative damage in rat tissues. Rats were randomly divided into six groups as follows; I-control group; II-methiocarb group; III-vitamin E group; IV-vitamin E
+
methiocarb group; V-taurine group and VI-taurine
+
methiocarb group. Methiocarb significantly increased lipid peroxidation in liver and kidney when compared to control groups. Levels of GSH and activities of SOD, CAT and GSH-Px were found to be decreased, while GSH-Rd remained unchanged in rat liver and kidney treated with methiocarb. Pretreatment of vitamin E and taurine resulted in a significant decrease on lipid peroxidation, alleviating effects on GSH and antioxidant enzymes. The degenerative histological changes were less in liver than kidney of rats treated with methiocarb. Pretreatment of vitamin E and taurine showed a protective effect on the histological changes in kidney comparing to the liver of rats treated with methiocarb.