Regulation of phenylpropanoid biosynthesis by MdMYB88 and MdMYB124 contributes to pathogen and drought resistance in apple

• Published in: Horticulture research Vol. 7; no. 1
• Main Authors: Geng, Dali, Shen, Xiaoxia, Xie, Yinpeng, Yang, Yusen, Bian, Ruiling, Gao, Yuqi, Li, Pengmin, Sun, Liying, Feng, Hao, Ma, Fengwang, Guan, Qingmei
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 2020; Oxford University Press
• Subjects: 631/449/2661/2146; 631/449/2661/2666; Accumulation; Agriculture; Apples; Biomedical and Life Sciences; Biosynthesis; Disease resistance; Drought; Drought resistance; Ecology; Electrophoretic mobility; Flavonoids; Hydrogen peroxide; Life Sciences; Lignin; Metabolites; Metabolomics; Pathogens; Phenylalanine; Phenylpropanoids; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Sciences; RNA-mediated interference; Roots; Stress; Transgenic plants
• ISSN: 2662-6810; 2052-7276
• DOI: 10.1038/s41438-020-0324-2

MdMYB88 and MdMYB124 have been demonstrated to be responsible for lignin accumulation in apple under drought stress. In this study, using a metabolomic approach, we identified differentially accumulated phenylpropanoid and flavonoid metabolites in
MdMYB88/124
transgenic RNAi plants under control and long-term drought stress conditions in apple roots. We confirmed the regulation of phenylalanine by MdMYB88 and MdMYB124 via UPLC-MS in apple roots under both control and drought conditions. Using Electrophoretic Mobility Shift Assay (EMSA) and ChIP-quantitative PCR (qPCR) analyses, we found that MdMYB88 positively regulates the MdCM2 gene, which is responsible for phenylalanine biosynthesis, through binding to its promoter region. Under long-term drought conditions,
MdMYB88/124
RNAi plants consistently accumulated increased amounts of H
2
O
2
and MDA, while
MdMYB88
and
MdMYB124
overexpression plants accumulated decreased amounts of H
2
O
2
and MDA. We also examined the accumulation of metabolites in the phenylpropanoid biosynthesis pathway in the leaves of
MdMYB88
and
MdMYB124
transgenic apple plants after long-term drought stress. We found that metabolites responsible for plant defense, including phenylpropanoids and flavonoids, accumulated less in the RNAi plants but more in the overexpression plants under both control and drought conditions. We further demonstrated that
MdMYB88/124
RNAi plants were more sensitive to
Alternaria alternata
f. sp.
mali
and
Valsa mali
, two pathogens that currently severely threaten apple production. In contrast,
MdMYB88
and
MdMYB124
overexpression plants were more tolerant to these pathogens. The cumulative results of this study provided evidence for secondary metabolite regulation by MdMYB88 and MdMYB124, further explained the molecular roles of MdMYB88 and MdMYB124 in drought resistance, and provided information concerning molecular aspects of their roles in disease resistance.