Alpha-amylase production by Bacillus subtilis CM3 in solid state fermentation using cassava fibrous residue

• Published in: Journal of basic microbiology Vol. 47; no. 5; pp. 417 - 425
• Main Authors: Swain, M.R, Ray, R.C
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01-10-2007; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: alpha-Amylases - biosynthesis; Bacillus subtilis; Bacillus subtilis - enzymology; Bacillus subtilis - growth & development; Bacillus subtilis - metabolism; Bacillus subtilis CM3; Bioreactors; Cassava fibrous residue; Fermentation; Manihot; Manihot esculenta; Response surface methodology; Solid state fermentation; Starch - metabolism; Tuber; α -Amylase
• ISSN: 0233-111X; 1521-4028
• DOI: 10.1002/jobm.200710132

In extraction of starch from cassava (Manihot esculenta Crantz), one of the major solid waste released is fibrous residues which constitute 15-20% by weight of the cassava chips/tuber processed. Production of α -amylase under solid state fermentation by Bacillus subtilis CM3 has been investigated using cassava fibrous residue. Response surface methodology (RSM) was used to evaluate the effect of the main variables, i.e. incubation period, initial medium pH, moisture holding capacity and temperature on enzyme production. A full factorial Central Composite Design (CCD) was applied to study these main factors that affected α -amylase production. The experimental results showed that the optimum incubation period, initial medium pH, moisture holding capacity and temperature were 6 days, 8.0, 70% and 50 °C, respectively. (© 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)