Putative Stress Sensors WscA and WscB Are Involved in Hypo-Osmotic and Acidic pH Stress Tolerance in Aspergillus nidulans

• Published in: Eukaryotic Cell Vol. 10; no. 11; pp. 1504 - 1515
• Main Authors: Futagami, Taiki, Nakao, Seiki, Kido, Yayoi, Oka, Takuji, Kajiwara, Yasuhiro, Takashita, Hideharu, Omori, Toshiro, Furukawa, Kensuke, Goto, Masatoshi
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01-11-2011
• Subjects: acidification; antifungal agents; Aspergillus nidulans; Aspergillus nidulans - genetics; Aspergillus nidulans - growth & development; Aspergillus nidulans - physiology; Cell Wall - metabolism; Cell Wall - physiology; cell walls; conidia; Echinocandins - pharmacology; Fungal Proteins - genetics; Fungal Proteins - metabolism; gene expression regulation; Gene Expression Regulation, Fungal; Gene Knockout Techniques; genes; Genes, Fungal; Glucans; Glucosyltransferases - biosynthesis; Glucosyltransferases - genetics; growth retardation; Hydrogen-Ion Concentration; hyphae; Lipopeptides - pharmacology; Membrane Proteins - metabolism; mitogen-activated protein kinase; Mitogen-Activated Protein Kinases - metabolism; Osmotic Pressure; phenotype; Phosphorylation; proteins; Saccharomyces cerevisiae; Sequence Alignment; Signal Transduction; Spores, Fungal - physiology; stress tolerance; Stress, Physiological - genetics; transcription (genetics); yeasts
• ISSN: 1535-9778; 1535-9786
• DOI: 10.1128/EC.05080-11

Wsc proteins have been identified in fungi and are believed to be stress sensors in the cell wall integrity (CWI) signaling pathway. In this study, we characterized the sensor orthologs WscA and WscB in Aspergillus nidulans. Using hemagglutinin-tagged WscA and WscB, we showed both Wsc proteins to be N- and O-glycosylated and localized in the cell wall and membrane, implying that they are potential cell surface sensors. The wscA disruptant (ΔwscA) strain was characterized by reduced colony and conidia formation and a high frequency of swollen hyphae under hypo-osmotic conditions. The deficient phenotype of the ΔwscA strain was facilitated by acidification, but not by alkalization or antifungal agents. In contrast, osmotic stabilization restored the normal phenotype in the ΔwscA strain. A similar inhibition was observed in the wscB disruptant strain, but to a lesser extent. In addition, a double wscA and wscB disruptant (ΔwscA ΔwscB) strain was viable, but its growth was inhibited to a greater degree, indicating that the functions of the products of these genes are redundant. Transcription of α-1,3-glucan synthase genes (agsA and agsB) was significantly altered in the wscA disruptant strain, resulting in an increase in the amount of alkali-soluble cell wall glucan compared to that in the wild-type (wt) strain. An increase in mitogen-activated protein kinase (MpkA) phosphorylation was observed as a result of wsc disruption. Moreover, the transient transcriptional upregulation of the agsB gene via MpkA signaling was observed in the ΔwscA ΔwscB strain to the same degree as in the wt strain. These results indicate that A. nidulans Wsc proteins have a different sensing spectrum and downstream signaling pathway than those in the yeast Saccharomyces cerevisiae and that they play an important role in CWI under hypo-osmotic and acidic pH conditions.