Preliminary results of SARS-CoV-2 detection in sewerage system in Niterói municipality, Rio de Janeiro, Brazil

This study presents preliminary results from a sewage-based surveillance to monitor the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the municipality of Niterói, State of Rio de Janeiro, Brazil. By using ultracentrifugation method associated to quantitative reverse trans...

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Bibliographic Details
Published in:	Memórias do Instituto Oswaldo Cruz Vol. 115; p. e200196
Main Authors:	Prado, Tatiana, Fumian, Tulio Machado, Mannarino, Camille Ferreira, Maranhão, Adriana Gonçalves, Siqueira, Marilda Mendonça, Miagostovich, Marize Pereira
Format:	Journal Article
Language:	English
Published:	Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01-01-2020 Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:	Betacoronavirus - isolation & purification Brazil Coronavirus Infections - epidemiology COVID-19 Humans Niterói Pandemics PARASITOLOGY Pneumonia, Viral - epidemiology Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 sewage Sewage - virology Short Communication TROPICAL MEDICINE Ultracentrifugation Brazil Niterói SARS-CoV-2 sewage
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Summary:	This study presents preliminary results from a sewage-based surveillance to monitor the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the municipality of Niterói, State of Rio de Janeiro, Brazil. By using ultracentrifugation method associated to quantitative reverse transcription polymerase chain reaction (RT-qPCR) we detected SARS-CoV-2 in 41.6% (5/12) of raw sewage samples obtained from sewage treatment plants and sewers network in the city. This pioneer study carried out in Brazil aims to subsidise information for health surveillance concerning the viral circulation in different areas of the city and, revealed the insertion and importance of environmental virology in health public policies.
Bibliography:	TP and TMF wrote the manuscript and contributed to the design and execution of the methodologies; CFM with the logistics of the monitored areas and sample collection; AGM carried out virus concentration; MMS with the editing and revision of the manuscript and MPM with the conception, coordination and supervision of research. All authors read and approved the final manuscript. The authors declare that they have no conflict of interest related to this work. TP and TMF contributed equally to this work.
ISSN:	0074-0276 1678-8060 1678-8060
DOI:	10.1590/0074-02760200196