Connexin32: a mediator of acetaminophen-induced liver injury?

• Published in: Toxicology mechanisms and methods Vol. 26; no. 2; pp. 88 - 96
• Main Authors: Maes, Michaël, McGill, Mitchell R., da Silva, Tereza Cristina, Lebofsky, Margitta, Maria Monteiro de Araújo, Cintia, Tiburcio, Taynã, Veloso Alves Pereira, Isabel, Willebrords, Joost, Crespo Yanguas, Sara, Farhood, Anwar, Zaidan Dagli, Maria Lucia, Jaeschke, Hartmut, Cogliati, Bruno, Vinken, Mathieu
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 12-02-2016
• Subjects: Acetaminophen; Acetaminophen - administration & dosage; Acetaminophen - metabolism; Acetaminophen - toxicity; Alanine Transaminase - blood; Animals; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - pathology; connexin32; Connexins - genetics; Connexins - metabolism; Cytokines - blood; gap junction; Gap Junction beta-1 Protein; Glutathione Disulfide - metabolism; hepatotoxicity; Liver - drug effects; Liver - immunology; Liver - metabolism; Liver - pathology; Male; Mice, Inbred C57BL; Mice, Knockout; Proliferating Cell Nuclear Antigen - metabolism; Protein Binding; gap junction; connexin32; Acetaminophen; hepatotoxicity
• ISSN: 1537-6516; 1537-6524
• DOI: 10.3109/15376516.2015.1103000

Connexin32 is the building block of hepatocellular gap junctions, which control direct intercellular communication and thereby act as goalkeepers of liver homeostasis. This study was set up to investigate whether connexin32 is involved in hepatotoxicity induced by the analgesic and antipyretic drug acetaminophen. To this end, whole body connexin32 knock-out mice were overdosed with acetaminophen followed by sampling at different time points within a 24-h time frame. Evaluation was done based upon a series of clinically and mechanistically relevant read-outs, including protein adduct formation, histopathological examination, measurement of alanine aminotransferase activity, cytokine production, levels of reduced and oxidized glutathione and hepatic protein amounts of proliferating cell nuclear antigen. In essence, it was found that genetic ablation of connexin32 has no influence on several key events in acetaminophen-induced hepatotoxicity, including cell death, inflammation or oxidative stress, yet it does affect production of protein adducts as well as proliferating cell nuclear antigen steady-state protein levels. This outcome is not in line with previous studies, which are contradicting on their own, as both amplification and alleviation of this toxicological process by connexin32 have been described. This could question the suitability of the currently available models and tools to investigate the role of connexin32 in acetaminophen-triggered hepatotoxicity.