Fate of di (2‑ethylhexyl) phthalate in different soils and associated bacterial community changes

• Published in: The Science of the total environment Vol. 637-638; no. C; pp. 460 - 469
• Main Authors: Zhu, Fengxiao, Zhu, Changyin, Doyle, Evelyn, Liu, Hailong, Zhou, Dongmei, Gao, Juan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-10-2018; Elsevier
• Subjects: Bacterial community response; Biodegradation; Diethylhexyl Phthalate - analysis; Metabolite; Models, Chemical; Phthalate ester; Phthalic Acids; Soil; Soil Microbiology; Soil Pollutants - analysis; Biodegradation; Phthalate ester; Bacterial community response; Metabolite
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2018.05.055

Di (2‑ethylhexyl) phthalate (DEHP) is a ubiquitous organic pollutant, which has caused considerable pollution in arable soils. In this study, the relationship between DEHP degradation potential and soil properties in 12 agricultural soils (S1-S12) was examined in a microcosm based experiment. Six of these soils were then selected to monitor patterns in bacterial community responses. It was found that DEHP degradation was positively correlated with bacterial counts in the original soils, suggesting a key role for bacteria in degradation. However, DEHP metabolism did not always lead to complete degradation. Its monoester metabolite, mono (2‑ethylhexyl) phthalate (MEHP), was present at appreciable levels in the two acidic soils (S1 and S2) during the incubation period of 35 days. Based on high-throughput sequencing data, we observed a greater impact of DEHP contamination on bacterial community structure in acidic soils than in the other soils. Nocardioides, Ramlibacter and unclassified Sphingomonadaceae were enriched in the two near-neutral soils where degradation was highest (S4 and S7), suggesting that these organisms might be efficient degraders. The relative abundance of Tumibacillus was greatly reduced in 50% of the six soils examined, demonstrating a high sensitivity to DEHP contamination. Furthermore, putative organic-matter decomposing bacteria (including Tumebacillus and other bacteria taxa such as members from Micromonosporaceae) were greatly reduced in the two acidic soils (S1 and S2), possibly due to the accumulation of MEHP. These results suggest a crucial role of soil acidity in determining the fate and impact of DEHP in soil ecosystems, which deserves further investigation. This work contributes to a better understanding of the environmental behavior of DEHP in soil and should facilitate the development of appropriate remediation technologies.
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•DEHP degradation positively correlated with bacterial numbers in 12 agricultural soils.•DEHP metabolites, including mono (2‑ethylhexyl) phthalate, 2‑ethylhexanoic acid, phthalic acid, protocatechuic acid and benzoic acid, were quantified.•Similar bacterial groups were enriched in the two soils displaying the greatest DEHP degradation.•Tumebacillus was particularly sensitive to DEHP.