Anthocyanins from Eugenia brasiliensis edible fruits as potential therapeutics for COPD treatment

• Published in: Food chemistry Vol. 134; no. 3; pp. 1256 - 1262
• Main Authors: Flores, Gema, Dastmalchi, Keyvan, Paulino, Sturlainny, Whalen, Kathleen, Dabo, Abdoulaye J., Reynertson, Kurt A., Foronjy, Robert F., D’Armiento, Jeanine M., Kennelly, Edward J.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-10-2012; Elsevier
• Subjects: Anthocyanins; Anthocyanins - pharmacology; Biological and medical sciences; Brazil; Cells, Cultured; Chromatography, High Pressure Liquid; COPD; Cyanidin; Delphinidin; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Eugenia - chemistry; Eugenia brasiliensis; Food industries; Fruit - chemistry; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Humans; IL-8; Interleukin-8 - metabolism; Mass Spectrometry; Myrtaceae; Plant Extracts - pharmacology; Pulmonary Disease, Chronic Obstructive - drug therapy; Respiratory System - drug effects; Respiratory System - metabolism; Smoke - adverse effects; Cyanidin; Delphinidin; Myrtaceae; Eugenia brasiliensis; Anthocyanins; IL-8; COPD; Anthocyanin; Fruit; Eugenia; Flavonoid; Polyphenol; Dicotyledones; Angiospermae; Pigments; Anthocyanidin; Spermatophyta
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.01.086

► Nine anthocyanins (1–9) were characterized from Eugenia brasiliensis edible fruit. ► Seven of them are reported for the first time in this plant. ► Delphinidin (8) and cyanidin (9), major constituents, were evaluated for their IL-8 inhibition in cells treated with CSE. ► Both anthocyanins were able to decrease IL-8 expression.
Nine anthocyanins (1–9) from the edible fruits of Eugenia brasiliensis were identified by HPLC-PDA and LC–MS, and seven of these are described for the first time in this Brazilian fruit. Two of the major anthocyanins, delphinidin (8) and cyanidin (9), were studied for their inhibitory activity against chemokine interleukin-8 (IL-8) production before and after cigarette smoke extract (CSE) treatment of cells. In non-treated cells the amount of IL-8 was unchanged following treatment with cyanidin and delphinidin in concentrations 0.1–10μM. Both delphinidin (8) and cyanidin (9) decreased the production of IL-8 in treated cells, at 1 and 10μM, respectively. Delphinidin (8) demonstrated IL-8 inhibition in the CSE treated cells in a dose-dependent manner.