Effects of Intracoronary Administration of Autologous Adipose Tissue-Derived Stem Cells on Acute Myocardial Infarction in a Porcine Model

• Published in: Yonsei medical journal Vol. 56; no. 6; pp. 1522 - 1529
• Main Authors: Lee, Hye Won, Lee, Han Cheol, Park, Jong Ha, Kim, Bo Won, Ahn, Jinhee, Kim, Jin Hee, Park, Jin Sup, Oh, Jun-Hyok, Choi, Jung Hyun, Cha, Kwang Soo, Hong, Taek Jong, Park, Tae Sik, Kim, Sang-Pil, Song, Seunghwan, Kim, Ji Yeon, Park, Mi Hwa, Jung, Jin Sup
• Format: Journal Article
• Language: English
• Published: Korea (South) Yonsei University College of Medicine 01-11-2015; 연세대학교의과대학
• Subjects: Adipose Tissue - cytology; Animals; Bone Marrow Cells - cytology; Bone Marrow Cells - metabolism; Chemokine CXCL12; Coronary Vessels; Female; Heart - physiopathology; Heart Ventricles; Mesenchymal Stromal Cells; Myocardial Infarction - diagnostic imaging; Myocardial Infarction - physiopathology; Myocardial Infarction - therapy; Original; Stem Cell Transplantation; Swine; Technetium Tc 99m Sestamibi - pharmacology; Tomography, Emission-Computed, Single-Photon - methods; Troponin T; Ventricular Function, Left; 의학일반; Myocardial infarction; angiogenesis; mesenchymal stem cells; 99mTc MIBI; left ventricle remodeling
• ISSN: 0513-5796; 1976-2437
• DOI: 10.3349/ymj.2015.56.6.1522

Adipose-derived stem cells (ADSCs) are known to be potentially effective in regeneration of damaged tissue. We aimed to assess the effectiveness of intracoronary administration of ADSCs in reducing the infarction area and improving function after acute transmural myocardial infarction (MI) in a porcine model.
ADSCs were obtained from each pig's abdominal subcutaneous fat tissue by simple liposuction. After 3 passages of 14-days culture, 2 million ADSCs were injected into the coronary artery 30 min after acute transmural MI. At baseline and 4 weeks after the ADSC injection, 99mTc methoxyisobutylisonitrile-single photon emission computed tomography (MIBISPECT) was performed to evaluate the left ventricular volume, left ventricular ejection fraction (LVEF; %), and perfusion defects as well as the myocardial salvage (%) and salvage index. At 4 weeks, each pig was sacrificed, and the heart was extracted and dissected. Gross and microscopic analyses with specific immunohistochemistry staining were then performed.
Analysis showed improvement in the perfusion defect, but not in the LVEF in the ADSC group (n=14), compared with the control group (n=14) (perfusion defect, -13.0±10.0 vs. -2.6±12.0, p=0.019; LVEF, -8.0±15.4 vs. -15.9±14.8, p=0.181). There was a tendency of reducing left ventricular volume in ADSC group. The ADSCs identified by stromal cell-derived factor-1 (SDF-1) staining were well co-localized by von Willebrand factor and Troponin T staining.
Intracoronary injection of cultured ADSCs improved myocardial perfusion in this porcine acute transmural MI model.