Biosorption of lead using immobilized Aeromonas hydrophila biomass in up flow column system: Factorial design for process optimization

• Published in: Journal of hazardous materials Vol. 177; no. 1; pp. 312 - 322
• Main Authors: Hasan, S.H., Srivastava, P., Talat, M.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15-05-2010; Elsevier
• Subjects: Adsorption; Aeromonas hydrophila; Aeromonas hydrophila - chemistry; Aeromonas hydrophila - metabolism; Applied sciences; Bed Depth Service Time (BDST) Model; Biodegradation, Environmental; Biological and medical sciences; Biomass; Biotechnology; Central Composite Design (CCD); Computer programs; Equipment Design; Exact sciences and technology; Fitness; Flow rate; Fundamental and applied biological sciences. Psychology; Lead - isolation & purification; Mathematical models; Methods. Procedures. Technologies; Optimization; Others; Pb(II); Pollution; Polymers; Software; Sorption; Sorption–desorption; Sulfones; Various methods and equipments; Water Pollutants, Chemical - isolation & purification; Aeromonas hydrophila; Pb(II); Central Composite Design (CCD); Bed Depth Service Time (BDST) Model; Sorption–desorption; Desorption; Sorption-desorption; Biomass; Modeling; Uptake; Composite material; Optimization; Design; Sorption; Biosorption; Lead; Software; Aqueous solution; Factorial design; Entrapped microorganism
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2009.12.034

Free and immobilized biomass of
Aeromonas hydrophila has been utilized for the removal of Pb(II) from aqueous solution. Fitness of Langmuir sorption model to the sorption data indicated the sorption was monolayer and uptake capacity of biomass was 163.9 and 138.88
mg/g for the free and immobilized biomass respectively. 85.38% Pb(II) removal was achieved at bed height of 19
cm and flow rate of 2
mL/min and BDST model was in a good agreement with the experimental results (
r
2
>
0.997). An attempt has been made to optimize the process conditions for the maximum removal using Central Composite Design with the help of Minitab
® 15 software and the result predicted by optimization plots was 88.27% which is close to the experimental data i.e. 85.38%. Sorption–desorption studies revealed that polysulfone immobilized biomass could reused up to 16 cycles and bed was completely exhaust after 33 cycles.